@article{Özlem ÇALKAN SAĞLAM_Hayri SAĞLAM_2020, title={The Hot Water Treatments Effect on Callus Regeneration in Grafted Cuttings for Some Grape Varieties And Rootstocks}, volume={4}, url={https://ispecjournal.com/index.php/ispecjas/article/view/37}, DOI={10.46291/ISPECJASvol4iss2pp1-10}, abstractNote={<p>In this study, it was aimed to determine the effect of hot water application for the elimination of Agrobacterium vitis on callus formation in grafted cuttings in the production of healthy vine saplings. In the study, 99R and Ramsey rootstocks cuttings and Sultani Çekirdeksiz and Yalova İncisi scions were used as materials. Cuttings and scions were grafted with omega grafting method than, hot water treatment was applied for 15, 30 and 45 minutes at 50 <sup>o</sup>C. Ramsey rootstock has been grafted with Sultani Çekirdeksiz, Sultani Çekirdeksiz and Yalova İncisi have been grafted on 99R rootstock. Afterwards, grafted cuttings were included in the standard grafted seedlings production program and callus formation was evaluated. Callus formations in grafted cuttings were evaluated after hot water application and growing for 21 days. It was determined that there were important differences between the combinations. In hot water application, callus formation seems to depend on rootstock and variety rather than application time.</p>}, number={2}, journal={ISPEC Journal of Agricultural Sciences}, author={Özlem ÇALKAN SAĞLAM and Hayri SAĞLAM}, year={2020}, month={Jun.}, pages={136–145} }