Istiklal University, Faculty of Tourism, Gastronomy and Culinary Arts, Kahramanmaraş
Abstract
This study aimed to comparatively evaluate the chemical composition, phenolic profile, antioxidant activity, and color characteristics of ‘Hicaznar’ (Punica granatum L.) fruits grown in four distinct ecological regions of Adana (İmamoğlu, Feke, Kozan, Sarıçam) in Türkiye. Sugar profiling revealed significant location-dependent differences, with the Kozan population exhibiting the highest glucose (6.64 g 100 g-1), fructose (7.59 g 100 g-1), and total sugar contents (15.12 g 100 g-1), whereas Sarıçam showed the highest sucrose concentration (0.83 g 100 g-1). Fructose and glucose were the dominant soluble sugars in ‘Hicaznar’ regardless of growing location, with Kozan samples. Citric and malic acids were the predominant organic acids in all samples, whereas succinic and ascorbic acids were detected at considerably lower concentrations. The highest citric acid content was recorded in Sarıçam (0.12 g 100 g-1), while Kozan exhibited the greatest malic acid concentration (0.11 g 100 g-1). Overall, the Kozan region demonstrated the most favorable sugar-acid balance, while regional ecological factors exerted a strong influence on the biochemical and physical properties of ‘Hicaznar’ fruit.
GÜNDEŞLİ, M. A. (2026). Biochemical Effects of Ecological Variation in ‘Hicaznar’ . ISPEC Journal of Agricultural Sciences, 10(3), 910–922. https://doi.org/10.5281/zenodo.21990622
📄Aarabi, A., Barzegar, M., Azizi, M.H., 2008. Effect of cultivar and cold storage of pomegranate (Punica granatum L.) juices on organic acid composition. ASEAN Food Journal, 15(1): 45–55.
📄Al-Maiman, S.A., Dilshad, A., 2002. Changes in physical and chemical properties during pomegranate (Punica granatum) fruit maturation. Food Chemistry, 76: 437-441.
📄Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D., Fuhrman, B., 2000. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. The American Journal of Clinical Nutrition. 71(5): 1062-1076.
📄Çelik, F., Gündoğdu, M., Zenginbal, H., 2019. Profile of organic acid and vitamin c in fruits of some pomegranate genotypes, Yüzüncü Yıl University Journal of Agricultural Sciences, 29(3):489-495.
📄Gil, M.I., Tomás-Barberán, F.A., Hess-Pierce, B., Holcroft, D.M., Kader, A.A., 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry. 48(10): 4581- 4589.
📄Gözlekçi, Ş., 1997. Investigations on Fertilization, Fruit Development and Maturation in Pomegranate (Punica granatum Cv. Hicaznar). PhD, Akdeniz University, Antalya-Turkey, 154p. (in Turkish)
📄Hernández, F., Legua, P., Martínez, R., Melgarejo, P., Martínez, J.J., 2014. Fruit quality characterization of seven pomegranate accessions (Punica granatum L.) grown in Southeast of Spain. Scientia Horticulturae, 175: 174–180.
📄Holland, D., Hatib, K., Bar-Ya’akov, I., 2009. Pomegranate: botany, horticulture, breeding. Horticultural Reviews, 35: 127–191.
📄Jurenka JS. 2008. Therapeutic applications of pomegranate (Punica granatum L.): a review. Alternative Medicine Review, 13(2):128-144.
📄Kalaycıoğlu, Z., Erim, F.B., 2017. Total phenolic contents, antioxidant activities, and bioactive ingredients of juices from pomegranate cultivars worldwide. Food Chemistry, 221: 496-507.
📄Kandylis, P., Kokkinomagoulos, E., 2020. Food Applications and Potential Health Benefits of Pomegranate and its Derivatives. Foods, 9(2):122.
📄Kulkarni, A.P., Aradhya, S.M., 2005. Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93(2): 319–324.
📄Kurt, H., Kurt, H., Şahin, G., 2013. A study of agricultural geography: pomegranate (Punica granatum L.) cultivation in Turkey. Marmara Geographical Review, (27): 551-574.
📄Lansky, E.P., Newman, R.A., 2007. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology, 109(2): 177–206.
📄Les, F., Arbonés-Mainar, J.M., Valero, M.S., López, V., 2018. Pomegranate polyphenols and urolithin A inhibit-glucosidase, dipeptidyl peptidase-4, lipase, triglyceride accumulation and adipogenesis related genes in 3T3-L1 adipocyte-like cells. Journal Ethnopharmacol, 220: 67–74.
📄Melgarejo, P., Salazar, D.M., Artes, F., 2000. Organic acids and sugars composition of harvested pomegranate fruits. European Food Research and Technology, 211: 185-190.
📄Mohammed, C., Mohamed, A., Ahmed, A., Mariod, A., 2020. Antioxidant and anti-diabetic activity of pomegranate (Punica granatum L.) leaves extracts. Foods Raw Mater, 8:329–836.
📄Nafees, M., Jafar Jaskani, M., Ahmad, I., Maryam, Ashraf, I., Maqsood, A., Ahmar, S., Azam, M., Hussain, S., Hanif, A., Chen, J.T., 2020. Biochemical analysis of organic acids and soluble sugars in wild and cultivated pomegranate germplasm based in Pakistan. Plants, 9(4): 493.
📄Noreen, S., Hashmi, B., Aja, P.M., Atoki, A.V., 2025. Phytochemicals and pharmacology of pomegranate (Punica granatum L.): nutraceutical benefits and industrial applications: a review. Frontiers in Nutrition, 12: 1528897.
📄Ozgen, M.A., Durgac, C., Sedat. S., Kaya, C., 2008. Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean in region of Turkey. Food Chemistry, 111(3): 703-706.
📄Poyrazoglu, E., Gokmen, Y., Artik, N., 2002. Organic acids and phenolic compounds in pomegranates (Punica granatum L.) grow in Turkey. Journal of Food Composition and Analysis Care, 5: 66-74.
📄Sakar, E., Vardin, H., Karaaslan, M. İlhan Odabaşıoğlu, M.I., 2023. Characterization of Pomological and Physicochemical Properties of Zivzik and Pervari Pomegranate Genotypes Grown in Siirt Province. Erzincan University Journal of Science and Technology, 16(3): 811-825.
📄Syed, D.N., Afaq, F., Mukhtar, H., 2007. Pomegranate derived products for cancer chemoprevention. Seminars in Cancer Biology, 17(5): 377-385.
📄Tarantino, A., Difonzo, G., Disciglio, G., Frabboni, L., Paradiso, V.M., Gambacorta, G., Caponio F., 2022. Fresh pomegranate juices from cultivars and local ecotypes grown in southeastern Italy: comparison of physicochemical properties, antioxidant activity and bioactive compounds. Journal of the Science of Food and Agriculture-Wiley Online, 102(3):1185-1192.
📄Velioğlu, S., Ünal, Ç., Cemeroğlu, B., 1997. Chemical characterization of pomegranate juice. Fruit Processing, 8:307- 310.
📄Viuda-Martos, M., Fernández-López, J., Pérez-Álvarez, J.A., 2010. Pomegranate and its many functional components as related to human health: A review. Comprehensive Reviews in Food Science and Food Safety, 9(6): 635-654.
📄Yu, M., Gouvinhas, I., Chen, J., Zhu, Y., Deng, J., Xiang, Z., 2024. Unlocking the therapeutic treasure of pomegranate leaf: a comprehensive review on phytochemical compounds, health benefits, and future prospects. Food Chemistry, 23:101587.
📄Zarfeshany, A., Asgary, S., Javanmard, S.H., 2014. Potent health effects of pomegranate. Advanced Biomedical Research, 3: 100.