Open Access

Effect of Pediococcus acidilactici on Fermentation Quality, Aerobic Stability and in Vitro Digestibility of Fodder Pea, Barley, and Mixed Silages

1 University of Qadisiyah, College of Biotechnology, Department of Agriculture Biotechnology, Diwaniyah
2 Kırşehir Ahi Evran University, Agriculture Faculty, Agricultural Biotechnology Department, Kırşehir

Abstract

This study aimed to evaluate the effects of Pediococcus acidilactici MF098795 strain (1×109 CFU g-1) as a silage inoculant on the fermentation characteristics, microbial population, in vitro digestibility, and aerobic stability of fodder pea (FP), barley (B), and fodder pea-barley mixture (FPB) silages. Six treatment groups were designed: control fodder pea (CFP), control barley (CB), control mixture (CFPB), and inoculated groups FPPA9, BPA9, and FPBPA9. Results indicated that inoculation with P. acidilactici improved the fermentation quality by lowering pH values and increasing lactic acid bacteria (LAB) counts, while reducing yeast and mold populations, particularly in BPA9 and FPBPA9 groups. Aerobic stability parameters, including pH₂ and CO₂ levels, were significantly reduced in the inoculated silages, suggesting enhanced resistance to aerobic spoilage. In vitro digestibility metrics such as organic matter digestibility (OMD), metabolizable energy based on gas production (MEGP), and ME based on OMD (MEOMD) were positively influenced by the inoculant. The highest improvements were observed in FPPA9 and BPA9 groups. In conclusion, the application of Pediococcus acidilactici MF098795 strain at 1×109 CFU g-1 effectively enhanced fermentation efficiency, microbial stability, and digestibility of silages, supporting its potential as a promising biological additive in forage preservation.

Keywords

How to Cite

ABDULKARIM ALSHAALAN, A. T. ., & FİLİK, A. G. (2025). Effect of Pediococcus acidilactici on Fermentation Quality, Aerobic Stability and in Vitro Digestibility of Fodder Pea, Barley, and Mixed Silages. ISPEC Journal of Agricultural Sciences, 9(3), 868–880. https://doi.org/10.5281/zenodo.16728949

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