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Tarım ve Orman Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Ankara
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Tarım ve Orman Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Ankara
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Ankara Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Ankara
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Ankara Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Ankara
Abstract
Callosobruchus chinenis and C. maculatus are among the most common and important species causing significant damage to stored pulses. This research were carried out to evaluate the efficacy of sulfuryl flüoride gas on the different ages of egg stage of Callosbruchus chinensis at Ankara University, Faculty of Agriculture, Department of Plant Protection, Stored Product Pests laboratory during 2020-2021. The efficacy of sulfuryl fluoride gas were determined on the 0-24, 24-48, 48-72 and 72-96 h egg stage for 24 h exposure at 25 oC and 75% relative humidity condition. Dose rates of sulfuryl flüoride in this research were 0, 10, 15, 20, 30, 40, 50, 60 and 120 g m-3. Fumigation using sulfuryl fluoride were carried out in gas-tight, with volume of 28.3 l Labconco® fumigation chambers. Eggs laid on the half chickpea seads glued onto the glass slide were counted and recorded according to the changing yhe colour to the milky white (alive) after 14-d from fumigation. Results showe that LD50 values changed depending on the age of egg satge, and the most tolerant age of egg stage considering LD50 values was calculated as 48-72 h old egg stage. Additionally, dose rate for 100% mortality for all ages of egg stage in the experiment were determined as 50 g m-3. Researh results showed that sulfuryl fluoride could be effective fumigant as an alternative to phosphine gas.
Keywords
Callosobruchus chinensis,sulfuryl flüoride,pest of stored pulses,egg,mortality
How to Cite
KAHRAMAN, E., AYYILDIZ, N., EMEKCİ, M., & FERİZLİ, A. G. (2025). Effectiveness of Sulfuryl Flüoride Against the Egg Stage of Callosobruchus chinensis (L.) (Coleoptera: Bruchidae). ISPEC Journal of Agricultural Sciences, 9(4), 1059–1070. https://doi.org/10.5281/zenodo.17427127
📄Adak, M.S., Güler, M., Kayan, N., 2010. Yemeklik baklagillerin üretimini artırma olanakları. Türkiye Ziraat Mühendisliği VII. Teknik Kongresi, Kongre Bildiriler Kitabı, 11-15 Ocak, Ankara, s. 329-341.
📄Aidbhavi, R., Pratap, A., Verma, P., Lamichaney, A., Bandi, S.M., Nitesh, S.D., Akram, M., Rathore, M., Singh, B., Singh, N.P., 2021.
📄Screening of wild Vigna accessions for resistance to three bruchid species. Journal of Stored Products Research, 93: 101864.
📄Aly, M.A.M., El-Sayed, F.M.A., El-Bishlawy, H.M.I., 2005. Damage and quantitative loss caused by Callosobruchus maculatus (Coleoptera: Bruchidae) to some cowpea and faba bean varieties. Egyptian Journal of Agricultural Research, 83: 563–581.
📄Anonim, 2012. Food and Agriculture Organization of the United Nations: Home, (www.faostat.org),(Accessed: 28.05.2025).
📄Arora, S., Srivastava, C., 2021. Locational dynamics of concentration and efficacy of phosphine against pulse beetle, Callosobruchus maculatus (Fab). Crop Protection, 143: 105475.
📄Baltaci, D., Klementz, D., Gerowitt, B., Drinkall, M.J., Reichmuth, C.H., 2009. Lethal effects of sulfuryl fuoride on eggs of different ages and other life stages of the ware-house moth Ephestia elutella (Hübner). Journal of Stored Products Research, 45: 19-23.
📄Baoua, I.B., Amadou, L., Margam, V., Murdock, L.L., 2012. Comparative evaluation of six storage methods for postharvest preservation of cowpea grain. Journal of Stored Products Research, 49: 171–175.
📄Bell, C.H., 2006. Factors affecting the efficacy of sulfuryl fluoride as a fumigant, in Proceedings of the 9th International Working Conference on Stored Product Protection, Brazil, pp. 519–526.
📄Bell, C.H., Savvidou, N., Wontner Smith, T.J., 1999. The toxicity of sulfuryl flüoride (Vikane) to eggs of insects pests of flour mills (Eds: J. Zuxun, L. Quan, L. Yongsheng, T. Xianchang, G. Lianhua). Stored Product Protection, Sichuan Publishing House of Science & Technology, Chengdu, Sichuan Province, PR China, pp. 345–350.
📄Bell, C.H., Drinkall, M.J., 2000. Sulfuryl fluoride to replace methyl bromide use in the flour milling industry. Annual International Conference on Methyl Bromide Alternatives and Emissions Reductions, Orlando, Florida, pp. 3.
📄Bell, C.H., 2000. Fumigation in the 21st century. Crop Protection, 19: 563–569.
📄Benhalim, H., Chaudhry, M.Q., Mills, K.A., Price, N.R., 2004. Phosphine resistance in stored-product insects collected from various grain storage facilities in Morocco. Journal of Stored Product Research, 40: 241–249.
📄Berhe, M., Subramanyam, B., Demissie, G., Chichaybelu, M., Abera, F.A., Mahroof, R., Harvey, J., 2024. Impact of storage technologies and duration on insect Pest population, post‐harvest losses, and seed quality of stored chickpea in Ethiopia. Pest Management Science, 80(2): 518-532.
📄Chayaprasert, W., Maier, D.E., Ileleji, K.E., Murthy, J.Y., 2009. Effects of weather conditions on sulfuryl fluoride and methyl bromide leakage during structural fumigation in a flourmill. Journal of Stored Product Research, 45: 1–9.
📄Doharey, R.B., Katiyar, R.N., Singh, K.M., 1983. Ecotoxicological studies on pulse beetles infesting green gram. Technical Bull Golden Technology, 21: 110-114.
📄Giga, D.P., Smith, R.H., 1983. Comparative life history studies of four Callosobruchus species infesting cowpeas with special reference to Callosobruchus rhodesianus (Pic) (Coleoptera: bruchidae). Journal of Stored Products Research, 19: 189–198.
📄Hagstrum, D.W., 1985. Preharvest infestation of cowpeas by the cowpea weevil (Coleoptera:Bruchidae) and population trends during storage. Journal of Economic Entomology, 78: 358–361.
📄Kenaga, E.E., 1957. Some biological, chemical and physical properties of sulfuryl fluoride as insecticidal fumigant. Journal of Economic Entomology, 50: 1–6.
📄Kenaga, E.E., 1961. Time, temperature and dosage relationships of several insecticidal fumigants. Journal of Economic Entomology, 54: 337-342.
📄LeOra Software, 1994. POLO-PC: a user’s guide to probit or logit analysis. LeOra Software, Berkeley, CA.
📄Mbata, G.N., Johnson, M., Phillips, T.W., Payton, M.E., 2005. Mortality of life stages of cowpea weevil (Coleoptera: Bruchidae) exposed to low pressure at different temperatures. Journal of Economic Entomology, 98: 1070–1075.
📄Meikle, R.W., Stewart, D., 1962. The residue potential of sulfuryl fluoride, methyl bromide, and methane sulfonyl fluoride in structural fumigations. Journal of Agricultural and Food Chemistry, 10: 393–397.
📄Meikle, R.W., Steward, D., Globus, O.A., 1963. Drywood termite metabolism of Vikane fumigant as showing by labelled pool technique. Journal of Agriculture, Food and Chemistry, 11: 229–230.
📄Nayak, M., Jagadeesan, R., 2024. Insect resistance to fumigants in postharvest commodity protection: monitoring and management. Control and Management of Pests in Stored Products, CRC Press, pp. 38-73.
📄Nayak, M.K., Jagadeesan, R., Pavic, H., Burrill, P.R., Daglish, G.J., 2025. Developing practical phosphine fumigation strategies to manage Callosobruchus maculatus F. in stored mungbeans: Laboratory and silo-scale evaluations. Journal of Stored Products Research, 111: 102556.
📄Nayak, M.K., Daglish, G.J., Phillips, T.W., Ebert, P.R., 2020. Resistance to the fumigant phosphine and its management in insect pests of stored products: a global perspective. Annual Review of Entomology, 65: 333–350.
📄Outram, I., 1967. Factors affecting the resistance of insect eggs to sulphuryl fluoride. II: the distribution of sulphuryl-35S fluoride in insect eggs after fumigation. Journal of Stored Products Research, 3: 353–358.
📄Reichmuth, Ch., Schneider, B., Drinkall, M.J., 1999. Sulfuryl fluoride (Vikane) against eggs of different age of the Indian meal moth Plodia interpunctella (Hübner) and the Mediterranean flour moth Ephestia kuehniella (Zeller). (Eds: J. Zuxun, L. Quan, L. Yongsheng, T. Xianchang, G. Lianhua). Stored Product Protection, Sichuan Publishing House of Science & Technology, Chengdu, Sichuan Province, PR China, pp. 416–422.
📄Reichmuth, Ch., Scho¨ ller, M., Dugast, J.F., Drinkall, M.J., 1997. On the efficacy of sulfuryl fluoride against stored-product pest moths and beetles. Printco Ltd., Nicosia, pp. 17–23.
📄Schneider, B.M., 1993. Characteristics and global potential of the insecticidal fumigant, sulfuryl fluoride. Cambridge, p. 193-198.
📄Schneider, B.M., Hartsell, P.L., 1999. Control of stored product pests with Vikane gas fumigant (Sulfuryl fluoride). (Eds: J. Zuxun, L. Quan, L. Yongsheng, T. Xianchang, G. Lianhua). Sichuan Publishing House of Science & Technology, Chengdu, Sichuan Province, PR China, pp. 406–408.
📄Sharma, T.R., Rana, J.C., Sharma, R., Rathore, R., Sharma, P.N., 2006. Genetic diversity analysis of exotic and Indian accessions of common bean (Phaseolus vulgaris L.) using RAPD markers. Indian Journal of Genetics and Plant Breeding, 66(4): 275–278.
📄Sharma, S.S., 1989. Review of literature of the losses caused by Callosobruchus species (Bruchidae: Coleoptera) during storage of pulses. Technical Bull Golden Technology, 22: 62-68.
📄Stewart, D., 1956. Sulfuryl fluoride-a new fumigant for control of the drywood termite, Kalotermes minor Hagen. Journal of Economic Entomology, 50: 7-11.
📄Su, N.Y., Osbrink, W.L.A., Scheffrahn, R.H., 1989. Concentration-time relationship for fumigant efficacy of sulfuryl flüoride against the Formosan subterranean termite (Isoptera, Rhinotermitidae). Journal of Economic Entomology, 82: 156-158.
📄Su, N.Y., Scheffrahn, R.H., 1990. Efficacy of sulfuryl fluoride against four beetle pests of museums (Coleoptera: Dermestidae, Anobiidae). Journal of Economic Entomology, 83: 879-882.
📄
📄Torres, E.B., N´obrega, R.S., Fernandes-Júnior, P.I., Silva, L.B., dos Santos Carvalho, G., Marinho, R.D.C.N., Pavan, B.E., 2016. The damage caused by Callosobruchus maculatus on cowpea grains is dependent on the plant genotype. Journal of the Science of Food and Agriculture, 96: 4276–4280.
📄Tsai, W.T., Mason, L.J., Chayaprasert, W., Maier, D.E., Ileleji, K.E., 2011. Investigation of fumigant efficacy in flour mills under realworld fumigation conditions. Journal of Stored Product Research, 47: 179–184.
📄Williams, L.H., Sprenkel, R.J., 1990. Ovicidal activity of sulfuryl fluoride to anobiid and lyctid beetle eggs of various ages. Journal of Entomological Science, 25: 366-375.
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Nurullah AYYILDIZ
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