1 Sirnak University, Faculty of Agriculture, Department of Field Crops, Sirnak
2 Dicle University, Vocational School of Agriculture, Department of Plant and Animal Production, Diyarbakır
Abstract
This study investigated the essential oil yield and chemical composition of different plant parts (leaf, flower and herbage) of Salvia officinalis L. and Salvia triloba cultivated under semi-arid conditions in Southeastern Türkiye. Plant material was obtained from the collection garden of Dicle University, Faculty of Agriculture. Essential oils were extracted by hydrodistillation using a Clevenger-type apparatus and analyzed by GC–mass spectrometry (GC/MS). Essential oil yield ranged between 1.00–1.68%, with the highest values recorded in flowers (1.68% in S. officinalis and 1.50% in S. triloba). Sixteen constituents (>1% GC area), representing 95.55–99.18% of the total oil, were identified. Monoterpenes were the dominant class (72.08–90.67%), while sesquiterpenes ranged between 8.51–26.74%. The main components of S. triloba were 1,8-cineole (up to 54.05%), camphor (17.66%) and β-pinene (14.44%), whereas S. officinalis were determined as the main components by α-thujone (31.98%), β-thujone (14.73%), 1,8-cineole (20.13%) and camphor (14.38%). Significant plant part–dependent variation was observed in essential oil composition. GGE biplot analysis explained 92.15% of total variation, confirming distinct clustering according to species and plant parts. It was determined that essential oil components varied depending on the species and harvested plant parts.
Keywords
Salvia sp.,sage,essential oil,plant part
How to Cite
KAMÇI, G., & TONÇER, Özlem. (2026). Chemical Composition of the Essential Oil from Different Plant Parts of Two Important Salvia Species Cultivated in Semi Arid Environment. ISPEC Journal of Agricultural Sciences, 10(2), 334–343. https://doi.org/10.5281/zenodo.19329079
📄Bernotienė, G., Nivinskienė, O., Butkienė, R., Mockutė, D., 2007. Essential oil composition variability in sage (Salvia officinalis L.). Chemija, 18(4): 38-43.
📄Boelens, M.H., 1986. The essential oil of spike Lavender lavandula latifolia Vill. (L. spica DC). perfum. Flavorist, 11: 43–63.
📄Celep, F., Doğan, M., 2023. The genus Salvia in Turkey: morphology, ecology, phytogeograpy, endemism and threat categories. In Medicinal and Aromatic Plants of Turkey (pp. 107-120). Cham: Springer International Publishing.
📄Celik, S.A., Ayran, İ., Asuman, K., Yüksel, K., 2018. Essential oil yield and compositions of sage (Salvia officinalis L.) cultivated in different province of Turkey. International Journal of Agriculture Environment and Food Sciences, 2(Special 1): 193-195.
📄Chalchat, J.C., Michet, A., Pasquier, B., 1998. Study of clones of Salvia officinalis L. yields and chemical composition of essential oil. Flavour and Fragrance Journal, 13(1): 68-70.
📄Dantas, L.B., Alcântara, I.S., Júnior, C.P.S., De Oliveira, M.R.C., Martins, A.O., Dantas, T.M., De Menezes, I.R.A., 2022. In vivo and in silico anti-inflammatory properties of the sesquiterpene valencene. Biomedicine and Pharmacotherapy, 153: 113478.
📄Delamare, A.P.L., Moschen-Pistorello, I.T., Artico, L., Atti-Serafini, L., Echeverrigaray, S., 2007. Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chemistry, 100(2): 603-608.
📄Dudai, N., Lewinsohn, E., Larkov, O., Katzir, I., Ravid, U., Chaimovitsh, D., Putievsky, E., 1999. Dynamics of yield components and essential oil production in a commercial hybrid sage (Salvia officinalis × Salvia fruticosa cv. Newe Ya'ar No. 4). Journal of Agricultural and Food Chemistry, 47(10): 4341-4345.
📄Figueiredo, A.C., Barroso, J.G., Pedro, L.G., Scheffer, J.J., 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23(4): 213-226.
📄Franz, C., 1993. Genetics. In R. K. M. Hay, P. G. Waterman (Eds.), Volatile Oil Crops: Their Biology, Biochemistry and Production (pp. 93–166). Harlow, UK: Longman.
📄Gali-Muhtasib, H., 2006. Anticancer and medicinal properties of essential oil and extracts of East Mediterranean sage (Salvia triloba). Advances in Phytomedicine, 2: 169-180.
📄Herraiz-Peñalver, D., Cases, M.Á., Varela, F., Navarrete, P., Sánchez-Vioque, R., Usano-Alemany, J., 2013, Chemical characterization of Lavandula latifolia Medik. essential oil from Spanish wild populations. Biochemical Systematics and Ecology, 46: 59–68.
📄Karik, U., Çinar, O., Tuncturk, M., Sekeroglu, N., Gezici, S., 2018. Essential oil composition of some sage (Salvia spp.) species cultivated in İzmir (Turkey) ecological conditions. Indian Journal of Pharmaceutical Education and Research, 52(4): 102-107.
📄Li, B., Zhang, C., Peng, L., Liang, Z., Yan, X., Zhu, Y., Liu, Y., 2015. Comparison of essential oil composition and phenolic acid content of selected Salvia species measured by GC-MS and HPLC methods. Industrial Crops and Products, 69: 329-334.
📄Muñoz-Bertomeu, J., Arrillaga, I., Segura, J., 2007. Essential oil variation within and among natural populations of Lavandula latifolia and its relation to their ecological areas. Biochemical Systematics and Ecology, 35: 479–488.
📄Nabi, M.H.B., Ahmed, M.M., Mia, M.S., Islam, S., Zzaman, W., 2025. Essential oils: advances in extraction techniques, chemical composition, bioactivities, and emerging applications. Food Chemistry Advances, 8:101048.
📄Nguyen, H.T., Radácsi, P., Rajhárt, P., Németh, É.Z., 2019. Variability of thujone content in essential oil due to plant development and organs from Artemisia absinthium L. and Salvia officinalis L. Journal of Applied Botany and Food Quality, 92: 100-105.
📄Perry, N.B., Anderson, R.E., Brennan, N.J., Douglas, M.H., Heaney, A.J., McGimpsey, J.A., Smallfield, B.M., 1999. Essential oils from Dalmatian sage (Salvia officinalis L.): variations among individuals, plant parts, seasons and sites. Journal of Agricultural and Food Chemistry, 47(5): 2048-2054.
📄Putievsky, E., Ravid, U., Dudai, N. 1986. The essential oil and yield components from various plant parts of Salvia fruticosa. Journal of Natural Products, 49(6): 1015-1017.
📄Rehman, R., Hanif, M.A., Mushtaq, Z., Al-Sadi, A.M., 2016. Biosynthesis of essential oils in aromatic plants: A review. Food Reviews International, 32(2): 117-160.
📄Russo, A., Formisano, C., Rigano, D., Senatore, F., Delfine, S., Cardile, V., Bruno, M., 2013. Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food Chemistry, Toxicol, 55: 42-47.
📄Salachna, P., Wesołowska, A., Meller, E., Piechocki, R., 2021. Mushroom waste biomass alters the yield, total phenolic content, antioxidant activity and essential oil composition of Tagetes patula L. Industrial Crops and Products, 171: 113961.
📄Salido, S., Altarejos, J., Nogueras, M., Sánchez, A., 2004. Chemical composition and seasonal variations of spike lavander oil from southern Spain. Journal of Essential Oil Research, 16: 206–210.
📄Santos-Gomes, P.C., Fernandes-Ferreira, M., 2001. Organ-and season-dependent variation in the essential oil composition of Salvia officinalis L. cultivated at two different sites. Journal of Agricultural and Food Chemistry, 49(6): 2908-2916.
📄Sharifi-Rad, M., Ozcelik, B., Altın, G., Daşkaya-Dikmen, C., Martorell, M., Ramírez-Alarcón, K., Sharifi-Rad, J., 2018. Salvia spp. plants from farm to food applications and phytopharmacotherapy. Trends in Food Science and Technology, 80: 242-263.
📄Toncer, O., Karaman, S., Kamci, G., Erdem, E., 2022. Yield and essential oil composition of Lippia citriodora HBK leaves and flowers in semi-arid conditions. Journal of Essential Oil Bearing Plants, 25(1): 9-19.
📄Veličković, D.T., Ranđelović, N.V., Ristić, M.S., Veličković, A.S., Šmelcerović, A.A., 2003. Chemical constituents and antimicrobial activity of the ethanol extracts obtained from the flower, leaf and stem of Salvia officinalis L. Journal of the Serbian Chemical Society, 68(1): 17-24.
📄Verma, R.S., Padalia, R.C., Chauhan, A., 2015. Harvesting season and plant part dependent variations in the essential oil composition of Salvia officinalis L. grown in northern India. Journal of Herbal Medicine, 5(3): 165-171.
📄Wu, Y.B., Ni, Z.Y., Shi, Q.W., Dong, M., Kiyota, H., Gu, Y.C., Cong, B., 2012. Constituents from Salvia species and their biological activities. Journal of Chemical Reviews, 112(11): 5967-6026.
📄Yang, R., Crossa, J., Cornelius, P.L., Burgueno, J., 2009. Biplot analysis of GEI effect. Crop Science, 49: 1564-1576.
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Özlem TONÇER2