This study was carried out to determine the effect of nitrogen levels on yield and some yield properties in Virginia tobacco. The experiment was conducted in Bornova ecological condition, during the growth season of 2019 as a pot experiment grown under outdoor. In the experiment, Virginia tobacco seedlings were planted with different fertilization levels of nitrogen (0, 30, 60, 90, 120, 150 kg ha^-1). Some parameters were evaluated in the study such as plant height (cm), number of the leaves (per plant^-1), leaf length (cm), leaf width (cm), stem diameter (mm), diameter rate, ovalite coefficiency, fresh and dry leaf yield (g plant^-1), total alkaloid (nicotine) (%). The effect of nitrogen levels were significant on all parameter tested except stem diameter and diameter rate. Based on these results, 120 kg ha^-1 N was proved the best fertilizer levels for Virginia tobacco under Bornova ecological conditions.