1
Gazi University, Department of Biology Education, Faculty of Gazi Education, Ankara
2
Gazi University, Department of Biology Education, Faculty of Gazi Education, Ankara
Abstract
Turkey is an important center of diversity for many plants species including bulbs, rhizomes, tubers, and other plants of high agricultural and horticultural importance. These species have a special importance as ornamental plants. However, due to urbanization and related factors, many of them are under threat. One of these species is the endemic Muscari racemosum Mill. The current study aimed to develop an efficient in vitro commercial bulblet propagation procedure using different explants. Twin-scale bulb explants were regenerated on MS medium having several doses of Kinetin+NAA (1-Naphthaleneacetic acid). The best regeneration was exhibited on 4.65 μM Kinetin+5.37 μM NAA at the end of 10 weeks with induction of 4.08 bulblets/explant with a mean diameter of 0.31 cm. The primary bulblets were cultured on MS medium having 18.60 μM Kinetin+5.37 μM NAA. About a 2.5-fold increase in the diameter of the bulbs (0.76 cm) was exhibited on the regenerated bulblets. The bulblets were regenerated on leaf bases taken from MS medium having several doses of BAP (6-Benzylaminopurine) + NAA. The regenerated bulbs were rooted on MS medium having 4.90 μM IBA (Indole-3-butyric acid) followed by their transference to a greenhouse for acclimatization. This study provided important information on commercial clonal propagation of M. racemosum and the importance of explants and growth regulators in plant regeneration.
Keywords
double scales, mass propagation, rooting, in vitro, acclimatization
How to Cite
Çiğdem Alev ÖZEL, & Fatma ÜNAL. (2021). In Vitro Regeneration of Muscari racemosum Mill. Using Twin Bulb Scales, Primary Bulbs, and Leaf Bases. ISPEC Journal of Agricultural Sciences, 5(3), 714–727. https://doi.org/10.46291/ISPECJASvol5iss3pp714-727
📄Al-Sammarraie, O.F.A. 2020. Muscari macrocarpum sweet ve Muscari racemosum Mill. türlerinin genotip ve sitotiplerinin kıyaslanması. Selçuk Üniversitesi, Yüksek Lisans Tezi, Fen Bilimleri Enstitüsü, Konya.
📄Altuntaş, A. 2020. Benefit from natural plants in landscape architecture: Example of Siirt Geophytes. ISPEC Journal of Agricultural Sciences, 4(2): 260-271.
📄Aslan, N. 1992. Doğal ekonomik bitkilerin korunması. Tarım ve Köy Dergisi, Sayı 74, Ankara.
📄Azad, M.A.K., Amin, M.N. 2012. Effects of the hormonal and basal nutrient medium on in vitro regeneration of an ornamental plant Muscari armeniacum Leichtlin. ex Baker. Plant Tissue Culture and Biotechnology, 22(2): 113-126.
📄Çetin, E.S., Kazaz, S., Baydar, N.G. 2007. Effects of different media on shoot tip culture of carnation (Dianthus caryophyllus L.). Derim,
📄Da Silva, J.A.T., Dobránszki, J. 2016. Tissue culture of Muscari species: present achievements and future perspectives. Rendiconti Lincei, 27(3): 427-441.
📄Demirci Kayiran, S., Özhatay, F.N. 2017. A karyomorphological study on the genus Muscari Mill. growing in Kahramanmaraş (Turkey). Turkish Journal of Botany, 41(3). 289-298
📄Eker, I. 2012. Muscari Mill. – In: Güner, A. [ed.], Türkiye bitkileri listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, pp. 98–100.
📄Eker, İ., Yıldırım, H., Armağan, M. 2019. Türkiye florası için yeni bir müşkürüm kaydı: Muscari pallens (M. Bieb.) Fisch (Kuşkonmazgiller/Asparaga ceae). Bağbahçe Bilim Dergisi, 6(1): 45-53.
📄Eker, I. 2019a. Muscari fatmacereniae (Asparagaceae, Scilloideae), a new species from southern Anatolia. Phytotaxa, 397(1): 99-106.
📄Eker, I. 2019b. Muscari pamiryigidii (Asparagaceae, Scilloideae), a new species from northwestern Anatolia. Phytotaxa, 408(4): 255-266.
📄Eker, I., Duman, H., Yıldırım, H. 2020a. Muscari muglaensis (Asparagaceae, Scilloideae), a new species from southwestern Anatolia. Phytotaxa, 475(4): 267-278.
📄Eker, I., Kandemir, A. 2020b. (Muscari sintenisii freyn (Asparagaceae)’nin taksonomik dirilişi ve türün lektotipifikasyonu. Bağbahçe Bilim Dergisi, 7(3): 12-24.
📄Eroğlu, H. 2020. Türkiye’de yayılış gösteren Muscari Mill. (Asparagaceae) cinsi taksonlarına ait morfoloji, palinoloji ve tohum yüzeyi araştırmaları. Doktora Tezi, Van Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Van.
📄Faraway, J.J. 2000. Practical regression and ANOVA using R. Vol. 168. Bath: University of Bath.
📄Faruq, M.O., Shahinozzaman, M., Azad, M.A.K., Amin, M.N. 2018. In vitro propagation of a cut flower variety Muscari armeniacum Leichtlin ex Baker through direct bulblet proliferation pathways. GSC Biological and Pharmaceutical Sciences, 5(1).
📄Fida, A. 2020. Van yöresinde yaygın olarak bulunan Muscari neglectum Guss’un in vitro koşullarda çoğaltımı. Yüksek Lisans Tezi, Van Yuzuncu Yıl Universitesi, Fen Bilimleri Enstitüsü, Van.
📄Govaerts, R. 2019. World checklist of Asparagaceae. Facilitated by the Royal Botanic Gardens, Kew. Retrieved April 15, 2019 from http://apps.kew.org/wcsp
📄Gürsoy, M. 2016. Muscari mirum speta (Asparagaceae) ve yakın akrabaları (Muscari massayanum Grunert, Muscari tenuiflorum Tausch ve Muscari latifolium Kirk)'nın morfolojik, anatomik ve ekolojik özellikleri. Doktora Tezi, Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü, Manisa.
📄Gürsoy, M., Şık, L. 2010. Comparative anatomical studies on Muscari armeniacum Leichtlin ex Baker and Muscari neglegtum Guss. In west Anatoli. Celal Bayar University Journal Of Science, 6(1): 61-72.
📄İlçim, A., Karataş, H., Karahan, F. 2020. Bazı muscari Mill. (Asparagaceae) türleri üzerine karşılaştırmalı morfolojik, anatomik ve palinolojik çalışmalar. Journal of the Institute of Science and Technology, 10(2): 846-854.
📄Jafari, A., Maassoumi, A.A. 2011. Synopsis of leopoldia, muscari and pseudomuscari (Hyacinthaceae) in Iran, with Leopoldia ghouschtchiensis sp. nova. In: Annales Botanici Fennici (48(5): 396-400). Finnish Zoological and Botanical Publishing Board.
📄Kayiran, S.D., Özhatay, N., Kaya, E. 2019. Muscari tauricum (Asparagaceae, Scilloideae), a new species from Turkey. Phytotaxa, 399(2): 109-118.
📄Kocak, M., Sevindik, B., Izgu, T., Tutuncu, M., Mendi, Y.Y. 2019. Synthetic seed production of flower bulbs. In Synthetic Seeds (pp. 283-299). Springer, Cham.
📄Meydan, İ. 2019. Badem (Amygdalus Trichamygdalus) meyvesinin etanol ekstraktı ve yağının Gs-Ms ile karakterizasyonu. Information Technology and Aplication Science, 14 (2): 241-250.
📄Mori, S., Nakano, M. 2004. Somatic embryo induction from leaf- and flower bud-derived calli in several Muscari species and cultivars. Propagation Ornamental Plants, 4(1): 58–62.
📄Nasırcılar, A.G., Mirici, S., Karagüzel, Ö, Eren, Ö., Baktir, İ. 2011. In vitro propagation of endemic and endangered Muscari mirum from different explant types. Turkish Journal of Botany, 35: 37–43.
📄Özdemir, F.A., Kılıç, Ö., Bağcı, E. 2017. In vitro bulb regeneration from stem explants of endemic geophyte Muscari aucheri (Boiss.) Baker. International Journal of Secondary Metabolite, 4(3 Special Issue 1): 50-54.
📄Ozel, Ç.A. 2008. Farklı Muscari türlerinin in vitro soğancık üretimi. Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
📄Ozel, C.A., Khawar, K.M., Unal, F. 2007. In vitro axillary bulblet regeneration of Turkish yellow grape hyacinth (Muscari macrocarpum Sweet) from twin scale explants. Research journal of agriculture and biological sciences, 3(6): 924-929.
📄Ozel, C.A., Khawar, K.M., Arslan, O., Unal, F. 2009. In vitro propagation of the golden grape hyacinth (Muscari macrocarpum Sweet) from twin scale explants. Propagation of Ornamental Plants, 9(4): 169-175.
📄Ozel, C.A., Khawar, K.M., Unal, F. 2015. Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale. Saudi Journal of Biological Sciences, 22(2): 132-138.
📄Ozel, Ç.A., Ünal, F. 2016. Efficient in vitro clonal propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron-α Naphthalene Acetic Acid. Turkish Journal of Agriculture-Food Science and Technology, 4(12): 1173-1178.
📄Şengün, İ.Y., Öztürk, B. 2018. Some natural antimicrobials of plant origin. Anadolu University Journal of Science and Technology C-Life Sciences and Biotechnology, 7 (2): 256-276.
📄Suzuki, S., Nakano, M. 2001. Organogenesis and somatic embryogenesis from callus cultures in Muscari armeniacum Leichtl. Ex Bak. In Vitro Cellular & Developmental Biology. 37: 382–387.
📄Uranbey, S., İpek, A., Caliskan, M., Dundar, E., Cocu, S., Basalma, D., Guneylioglu, H. 2010. In vitro bulblet induction from bulb scales of endangered ornamental plant Muscari azureum. Biotechnology Biotechnological Equipment, 24(2): 1843-1848.
📄Uranbey, S. 2010a. In vitro bulblet regeneration from immature embryos of Muscari azureum. African Journal of Biotechnology, 9(32): 5121-5125.
📄Uranbey, S. 2010b. Stimulating effects of different basal media and cytokinin types on regeneration of endemic and endangered Muscari aucheri. Archives Biology of Science, 62(3): 663-667.
📄Uzun, S., Parmaksiz, I., Uranbey, S., Mirici, S., Sarıhan, E.O., İpek, A., Özcan, S. 2014. In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik. Turkish Journal of Biology, 38(1): 83-88.
📄Van Scheepen, J. 1991. International checklist for hyacinths and miscellaneous bulbs. Royal General Bulb Growers’ Association (KAVB). Hillegom, The Netherlands, p. 409.
📄Vaziri, P. A., Uranbey, S., Sancak, C. 2014. Efficient in vitro micropropagation for the conservation of endemic and endangered aucher-eloy grape hyacinth [Muscari aucheri (Boiss.) Baker]. Journal of Applied Biological Sciences, 8(1): 80-83.
📄Wang, S., Yang, F., Jiu, L., Zhang, W., Zhang, W., Tian, Z., Wang, F. 2013. Plant regeneration via somatic embryogenesis from leaf explants of Muscari armeniacum. Biotechnology & Biotechnological Equipment, 27(6): 4243-4247.
📄Yıldırım, Ö. 2020. Keşişbaşı’nın (Muscari azureum Fenzl) tohumla çoğaltılması üzerine araştırmalar. Yüksek Lisans Tezi, Ahievran Üniversitesi, Fen Bilimleri Enstitüsü, Kırşehir.
📄Yildirimli, S. 2010. Some new taxa, records, and taxonomic treatments from Turkey. The Herb Journal of Systematic Botany, 17(2): 1-114.
📄Yücesan, B.B., Cicek, F., Gürel, E. 2014. Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker). Turkish journal of agriculture and forestry, 38(5): 716-722.
