1
Bingol University, Institute of Sciences, Bee and Bee product, Bingol
2
Bingol University, Faculty of Agriculture, Plant Protection, Bingol
3
Bingol University, Faculty of Agriculture, Agricultural Economics, Bingol
4
Bingol University, Faculty of Agriculture, Plant Protection, Bingol
Abstract
Fusarium spp is one of the major phytopathogenic microfungus strains causing severe losses in many economically cultivated crops. The soil-borne pathogen Fusarium solani has historically been considered a serious agent across the globe, causing vascular wilt and root rot in agroeconomic crops and eventually leading to plant death. Three different concentrations (1 µl, 2 µl, and 4 µl) of essential oils (EO) extracted from lavender (Lavandula officinalis L.) and summer savory (Satureja hortensis L.) plants were mixed separately with PDA medium, and their antifungal effect against F. solani was investigated in vitro. When the results of the experiment were evaluated statistically, it was determined that the increasing concentrations of summer savory essential oil repressed the mycelial growth of the fungus, while lavender oil did not have any positive or negative effects. The inhibition activity of summer savory EO on F. solani was calculated as 43, 53, and 90% at the concentrations of 1, 2, and 4 µl, respectively. In this study, it was found that summer savory EO, even at a minimum dose, had a negative effect on agriculturally important wilt agent. In this context, it can be asserted that summer savory EO is a promising natural substance for the development of various fungicide solutions to prevent fungal diseases caused by vascular origin.
ÇAKAR, G. ., SARAÇ SİVRİKAYA, I. ., KARAKAYA, E. ., & GÜLLER, A. . (2021). Evaluation of the In Vitro Fungicidal Activity of Summer Savory and Lavender Essential Oils Against Fusarium solani. ISPEC Journal of Agricultural Sciences, 5(4), 795–805. https://doi.org/10.46291/ISPECJASvol5iss4pp795-805
📄Abedi-Tizaki M, Zafari D, Sadeghi J (2016). First report of Fusarium solani causing stem rot of Dracaena in Iran. Journal of Plant Protectıon Research, 56 (1). DOI: 10.1515/jppr-2016-0013
📄Al –Naser Z, Al- Abrass N (2014). Chemical composition and fungitoxic activities of Lavandula officinalis L. oil and comparison with synthetic fungicide on the growth some fungi in vitro. International Journal of ChemTech Research, 6 (11): 4918-4926.
📄Al-Howiriny, TA (2003). Composition and antimicrobial activity of essential oil of Salvia lanigera. Pakistan Journal of Biological Sciences, 6(2): 133-135.
📄Al-Sadi AM, Al-Ghaithi AG, Al-Fahdi N, Al-Yahyai R (2014). Characterization and pathogenicity of fungal pathogens associated with root diseases of citrus in Oman. Int J Agric Biol, 16:371–376
📄Angioni A, Barra A, Coroneo V, Dessi S, Cabras P (2006). Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. J. Agric. Food Chem, 54(12): 4364–4370.
📄Astani A, Reichling J, Schnitzler P (2011). Screening for Antiviral Activities of Isolated Compounds from Essential Oils. Evidence-Based Complementary and Alternative Medicine, doi:10.1093/ecam/nep187
📄Bammi J, Khelifa R, Remmal A (1997). Etudes de l’activité antivirale de quelques huiles essentielles. In Proceedings of the Intern. Congr. Arom. Medicinal Plants & Essential Oils.
📄Bassolé IHN, Juliani HR (2012). Essential Oils in Combination and Their Antimicrobial Properties. Molecules. 17, 3989-4006. https://doi.org/10.3390/molecules17043989
📄Bayaz M (2014). Esansiyel Yağlar: Antimikrobiyal, Antioksidan ve Antimutajenik Aktiviteleri. Akademik Gıda 12(3): 45-53.
📄Baytop T (1999). Türkiye’de Bitkilerle Tedavi. Nobel Tıp Kitapevleri ISBN: 975-420-021-1 S: 332.
📄Benjilali B, Tantaoui EA, Ayadi A, Ihlal M (1984). Method to study antimicrobial effects of essential oils: application to theantifungal activity of six Moroccan essences. J. Food Prot. 47, 748–752.
📄Bishop CD (1995). Antiviral activity of the essential oil of Melaleuca alternifolia (Maiden and Betche) Cheel (tea tree) against tobacco mosaic virus. Journal of Essential Oil Research 7(6): 641- 644.
📄Brayford D (1993). The identification of Fusarium species. In: Workshop Manual. International Mycological Institute, Bakeham Lane, Egham. CAB International, Wallingford.
📄Bueno CJ, Fischer IH, Rosa DD, Firmino AC, Harakava R, Oliveira CMG, Furtado EL (2014). Fusarium solani f. sp. passiflorae: a new forma specialis causing collar rot in yellow passion fruit. Plant Pathol 63:382–389
📄Ceylan A (1983). Tıbbi Bitkiler-II. Ege Üniversitesi Ziraat Fakültesi Yayını No:481, Bornova-İzmir.
📄Ceylan A (1987). Tıbbi Bitkiler 2 (Uçucu Yağ İçerenler), Ege Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, 481:188, İzmir.
📄Choi HJ (2018). Chemical Constituents of Essential Oils Possessing Anti-Influenza A/WS/33 Virus Activity Osong Public Health and Research Perspectives. 9(6):348−353.
📄Chouhan S, Sharma K, Guleria S (2017). Antimicrobial Activity of Some Essential Oils-Present Status and Future Perspectives. Medicines (Basel). 4(3):58. doi:10.3390/medicines4030058
📄Cowan MM (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews 12(4): 564- 582.
📄Çelik E, Çelik GY (2007). Bitki Uçucu Yağlarının Antimikrobiyal Özellikleri. Orlab On-Line Mikrobiyoloji Dergisi. 5 (2): 1-6.
📄Dadalioglu I, Evrendilek G (2004). Chemical Compositions and Antibacterial Effects of Essential Oils of Turkish Oregano ( Origanum minutiflorum ), Bay Laurel ( Laurus nobilis ), Spanish Lavender ( Lavandula stoechas L.), and Fennel ( Foeniculum vulgare ) on Common Foodborne Pathogens. Journal of agricultural and food chemistry. 52. 8255-60. 10.1021/jf049033e.
📄Deans SG, Sobada KP (1990). Antimicrobial Properties of Marjoram (Origanum marjorana L.) Volatile Oil. Flavour Fragr. J. 5, 187-190.
📄Dikbas N, Kotan R, Dadasoglu F, Sahin F (2008). Control of Aspergillus flavus with essential oil and methanol extract of Satureja hortensis. International Journal of Food Microbiology. 124 (2): 179-182.
📄Djenane D, Yangüela J, Derriche F, Bouarab L, Roncales P (2012). Olive tree leaf extract; in vitro tests on staphylococcus aureus, salmonella enterditis and pseudomonas aeruginosa; application in turkey meat. Phytothérapie, 10, 10–18.
📄Djihane B, Wafa N, Elkhamssa S, Pedro DHJ, Maria EA, Mihoub ZM (2017). Chemical constituents of Helichrysum italicum (Roth) G. Don essential oil and their antimicrobial activity against Gram-positive and Gram-negative bacteria, filamentous fungi and Candida albicans, Saudi Pharmaceutical Journal, 25 (5): 780-787.
📄El-Shazly A, Dorai G, Wink M (2002). Composition and Antimicrobial Activity of Essential essential oils. Int. J. Food Microbiol. 122(1-2): 135– 139.
📄Farahani-Kofoet RD,Witzel K, Graefe J, Grosch R, Zrenner R (2020). Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus. Pathogens. 9, 509. doi:10.3390/pathogens9060509.
📄Gadisa E, Weldearegay G, Desta K, Tsegaye G, Hailu S, Jote K, Takele A (2019). Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria. BMC Complement Altern Med. 19 (24) https://doi.org/10.1186/s12906-019-2429-4
📄Gandomi H, Misaghi A, Basti AA, Bokaei S, Khosravi A, Abbasifar A, Javan AJ (2009). Effect of Zataria multiflora Boiss. essential oil on growth and aflatoxin formation by Aspergillus flavus in culture media and cheese. Food Chem. Toxicol. 47(10): 2397–2400.
📄Giordani R, Regli P, Kaloustian J, Mikaïl C, Abou L, Portugal H (2004). Antifungal effect of various essential oils against candida albicans. Potentiation of antifungal action of amphotericin B by essential oil from Thymus vulgaris. Phytotherapy Research, 18, 990–995.
📄Grassmann J, Elstner EF (2003). Essential oils/properties and uses. Encyclopaedia of Food Science, Food Technology and Nutrition (Elsevier Science Ltd.): 2177-2184 p.
📄Guynot ME, Ramos AJ, Seto L, Purroy P, Sanchis V, Marin S (2003). Antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. J. Appl. Microbiol. 94(5): 893–899.
📄György É, Laslo É, Kuzman IH, András CD (2020). The effect of essential oils and their combinations on bacteria from the surface of fresh vegetables. Food Science & Nutrition. 8: 5601–5611.
📄Hadian J, Ghasemnezhad M, Ranjbar H, Frazane M, Ghorbanpour M (2008). Antifungal potency of some essential oils in control of postharvest decay of strawberry caused by botrytis cinerea, rhizopus stolonifer and Aspergillus niger. Journal of Essential Oil Bearing Plants, 11, 553-562.
📄Hafizi R, Salleh B, Latiffah Z (2013). Morphological and molecular characterization of Fusarium. solani and F. oxysporum associated with crown disease of oil palm. Brazilian Journal of Microbiology 44, 3, 959-968
📄Kadoglidou K, Lagopodi A, Karamanoli K, Vokou D, Bardas GA, Menexes G, Constantinidou HIA (2011). Inhibitory and stimulatory effects of essential oils and individual monoterpenoids on growth and sporulation of four soil-borne fungal isolates of Aspergillus terreus, Fusarium oxysporum, penicillium expansum, and Verticillium dahliae. European Journal of Plant Pathology, 130, 297–309.
📄Kılıç A. (2008). Uçucu Yağ Elde Etme Yöntemleri. Bartın Orman Fakültesi Dergisi. 10 (13): 37-45.
📄Klaric MS, Kosalec J, Mastelic J, Pieckova E, Pepeljnak S (2007). Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings. Lett. Appl. Microbiol. 44(1): 36-42.
📄Kumar R, Mishra AK, Dubey NK, Tripathi YB (2007). Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Int. J. Food Microbiol. 115(2): 159–164.
📄Kurt Ş, Uysal A, Soylu EM, Kara M, Soylu S (2020). Characterization and pathogenicity of Fusarium solani associated with dry root rot of citrus in the eastern Mediterranean region of Turkey. Journal of General Plant Pathology. 86: 326–332
📄Lee SO, Choi GJ, Jang KS, Lim HK, Cho KY, Kim J (2007). Antifungal Activity of Five Plant Essential Oils as Fumigant Against Postharvest and Soilborne Plant Pathogenic Fungi. Plant Pathol. Journal, 23 (2): 97-102.
📄Limoncu ME, Balcıoğlu İC, Oyur T, Zeybek G, Zeybek U (2017). In vitro Investigation of the Pediculicidal Activities of the Volatile Oil Components of Some Medical Plants Raised in Turkey. Türkiye Parazitol Derg. 41: 208-213.
📄Linskens HF, Jackson JF (1997a) Modern Methods of Plant Analysis, Vol. 19: Plant Volatile Analysis, Springer, Germany.
📄Lopez-Malo A, Barreto-Valdivieso J, Palou E, San Martin F (2007). Aspergillus flavus growth response to cinnamon extract and sodium benzoate mixtures. Food Control 18(11): 1358-1362.
📄Lopez-Reyes JG, Spadaro D, Gullino ML, Garibaldi A (2010). Efficacy of plant essential oils on postharvest control of rot caused by fungi on four cultivars of apples in vivo. Flavour and Fragrance Journal, 25, 171–177.
📄Mafakheri H, Mirghazanfari SM (2018). Antifungal activity of the essential oils of some medicinal plants against human and plant fungal pathogens. Cell Mol Biol.v64(15): 13-19.
📄Man A, Santacroce L, Jacob R, Mare A, Man L. (2019). Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study. 25; 8(3) doi:10.3390/pathogens8010015
📄Nguefack J, Dongmo JBL, Dakole CD, Leth V, Vismer HF, Pedersen JGT, Guemdjom EFN, Mbeffo M, Tamgue O, Fotio D, Zollo PH, Nkengfack AE (2009). Food preservative potential of essential oils and fractions from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against mycotoxigenic fungi. Int. J. Food Microbiol.131(2-3): 151–156.
📄Nguefack J, Dongmo JBL, Dakole CD, Leth V, Vismer HF, Pedersen JGT, Guemdjom EFN, Mbeffo M, Tamgue O, Fotio D, Zollo PH, Nkengfack AE (2009). Food preservative potential of essential oils and fractions from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against mycotoxigenic fungi. Int. J. Food Microbiol.131(2-3): 151–156.
📄Nikolis M, Jovanovis KK, Markovis T, Markovis D, Gligorijevis N, Radulovis S, Sokovis M (2014). Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. Industrial Crops and Products, 61, 225–232.
📄Nostro A, Germano MP, D’angelo V, Marino A, Cannatelli MA, (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol. 30(5): 79-84.
📄O’Donnell K, Ward TJ, Robert VARG, Crous PW, Geiser DM, Kang S (2015). DNA sequence-based identification of Fusarium: current status and future directions. Phytoparasitica 43:583–595
📄Ondřej M, Dostálová R, & Trojan R (2008). Evaluation of virulence of Fusarium solani isolates on pea. Plant Protection Science, 44(No. 1), 9–18. doi:10.17221/519-pps
📄Ondřej M, Dostálová R, Trojan R (2008). Evaluation of virulence of Fusarium solani isolates on pea. Plant Protect. Sci., 44: 9–18.
📄Özcan M, Boyraz N (2000). Antifungal properties of some herb decoctions. Eur Food Res Technol (2000) 212: 86–88.
📄Özgüven M, Kırıcı S (1999). Farklı ekolojilerde Nane (Mentha) türlerinin verim ile uçucu yağ oran ve bilesenlerin arastırılması. Tr. J. of Agriculture and Foresty 23: 465-472.
📄Özkalp B. and Özcan MM (2009). Antibacterial Activity of Several Concentrations of Sater (Satureja hortensis L.) Essential Oil on Spoilage and Pathogenic Food - Related Microorganisms. World Applied Sciences Journal 6 (4): 509-514, ISSN 1818-4952.
📄Pinto E, Salgueiro LR, Cavaleiro C, Palmeira A, Gonzalves MJ (2007). In vitro susceptibility of some species of yeasts and filamentous fungi to essential oils of Salvia officinalis. Ind. Crop. Prod. 26(2): 135-141.
📄Ramteke P, Ghule M, Ramteke S (2019). First Report of Fusarium solani Causing Root Rot on Fenugreek (Trigonella foenum-graecum L.) in India. Plant Disease. 104. 10.1094/PDIS-08-19-1622-PDN.
📄Ramteke PK, Ghule MR, Ramteke, SD (2019). First Report of Fusarium solani Causing Root Rot on Fenugreek (Trigonella foenum-graecum L.) in India. Plant Disease. doi:10.1094/pdis-08-19-1622-pdn
📄Rasooli I, Fakoor MH, Yadegarinia D, Gachkar L, Allameh A, Rezaei MB (2008). Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. Int. J. Food Microbiol. 122(1-2): 135– 139.
📄Rasooli I, Rezaei MB, Alameh A (2006). Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock. Food Control 17(5): 359-364.
📄Rasooli I, Rezaei MB, Alameh A (2006). Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock. Food Control 17(5): 359-364.
📄Romberg MK and Davis RM 2007. Host range and phylogeny of Fusarium solani f. sp. eumartii from potato and tomato in California. Plant Dis. 91:585-592.
📄Rota C, Carramiñana JJ, Burillo J, Herrera A (2004). In vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens. Journal of Food Protection, 67: 1252- 1256.
📄Sahin F, Güllüce M, Daferera D, Sökmen A, Sökmen M., Polissiou M, Ağar G, Özer H (2004). Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. Vulgare in the Eastern Anatolia region of Turkey. Food Control 15(7): 549–557.
📄Saraç Sivrikaya I, Tosun B, Karakaya E (2021). Origanum onites L. ve Rosmarinus officinalis L. Uçucu Yağlarının Kimyasal İçeriklerinin ve Fusarium solani’ ye Karşı Antifungal Aktivitelerinin Belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi 8(2): 329–335.
📄Saremi H, Amiri ME, Ashrafi J (2011). Epidemiological aspects of bean decline disease caused by Fusarium species and evaluation of the bean resistant cultivars to disease in Northwest Iran. African Journal of Biotechnology 10(66): 14954-14961.
📄Sartoratto A, Machado ALM, Delarmelina C, Figueria GM, Duarte MCT, Rehder VLG (2004). Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian Journal of Microbiology 35(4): 275-280.
📄Schelz Z, Molnar J, Hohmann J (2006). Antimicrobial and antiplasmid activities of essential oils. Fitoterapia 77(4): 279-285.
📄Silva JKRD, Figueiredo PLB, Byler KG, Setzer WN (2020). Essential Oils as Antiviral Agents. Potential of Essential Oils to Treat SARS-CoV-2 Infection: An In-Silico Investigation. Int J Mol Sci. 21(10):3426.
📄Śmigielski KB, Prusinowska R, Krosowiak K, Sikora M (2013). Comparison of qualitative and quantitative chemical composition of hydrolate and essential oils of lavender (Lavandula angustifolia). J Essent Oil Res. 25: 291- 299.
📄Soylu EM, Kurt S, Soylu S (2010). In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent botrytis cinerea. International Journal of Food Microbiology, 143, 183–189.
📄Soylu EM, Soylu S, Kurt S (2006). Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopathologia, 161, 119–128.
📄Soylu EM, Yiğitbaş H, Tok MF, Soylu S, Baysal O, Kaya AD (2005). Chemical Composition and Antifungal Activity of the Essential Oil of Artemisia annua L. Against Foliar and Soilborne Fungal Pathogens. Z. Pflanzenk Pflanze, 112: 229-239.
📄Sugiura Y, Sugita-Konishi Y, Kumagai S, Reiss E (2003). Experimental murine hyalohyphomycosis with soil-derived isolates of Fusarium solani. Med Mycol. Jun;41(3):241-7. doi: 10.1080/13693780310001597377. PMID: 12964716.
📄SugiuraY., Sugita-Konishi Y., Kumagai S., Reis E (2003). Experimental murine hyalohyphomycosis with soil-derived isolates of Fusarium solani. Medical Mycology. 41, 241/247.
📄Şahin F, Karaman İ, Güllüce M, Öğütçü H, Şengül M, Adıgüzel A, Öztürk S, Kotan R (2003). Evaluation of antimicrobial activities of Satureja hortensis L. Journal of Ethnopharmacology. 87 (1): 61-65.
📄Tarek N, Hassan HM, AbdelGhani SMM, Radwan IA, Hammouda O, El-Gendy AO (2014). Comparative chemical and antimicrobial study of nine essential oils obtained from medicinal plants growing in egypt. Beni-Suef University Journal of Basic and Applied Sciences, 3, 149–156.
📄Tutun H, Koç N, Kart A (2018). Plant Essential Oils Used Against Some Bee Diseases. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 6(1), 34 - 45. Doi: 10.24925/turjaf.v6i1.34-45.1502
📄Tuxbury KA, Shaw GC, Montali RJ, Clayton LA, Kwiatkowski NP, Dykstra MJ, Mankowski JL (2014). Fusarium solani species complex associated with carapace lesions and bronchitis in captive American horseshoe crabs Limulus polyphemus. Dis Aquat Organ 109:223–230.
📄Tzortzakis NK, Economakis CD (2007). Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Innovative Food Sci. And EmergingTech. 8(2): 253–258.
📄Usanmaz Bozhuyuk A, Komaki A, Kordali S, Ustuner T (2019). Assessment of the growth inhibiting effect of Satureja essentiel oils on different Fusarium speceis from Wheat. Fresenius Environmental Bulletin. 28 (11): 8199-8206.
📄Usanmaz Bozhüyük A, Kordali Ş, Bölük G (2015). Satureja hortensis L. Uçucu Yağının Antifungal Etkisi Atatürk Univ. J. of the Agricultural Faculty, 46 (2): 107-112
📄Varona S, Rodríguez-Rojo S, Martín Á, Cocero MJ, Serra AT, Crespo T, Duarte CMM (2013). Antimicrobial activity of lavandin essential oil formulations against three pathogenic food-borne bacteria. Industrial Crops and Products, 42, 243–250.
📄Vazquez BI, Fente C, Franco CM, Vazquez MJ, Cepeda A (2001). Inhibitory effects of eugenol and thymol on Penicillium citrinum strains in culture media and cheese. Int. J. Food Microbiol. 67(1-2): 157–163.
📄Viuda-Martos M, Mohamady MA, Fernández-López J, Abd ElRazik KA, Omer EA, Pérez-Alvarez JA, Sendra E (2011). In vitro antioxidant and antibacterial activities of essentials oils obtained from egyptian aromatic plants. Food Control, 22, 1715–1722.
📄Wang R, Wang R, Yang B (2009). Extraction of essential oils from five cinnamon leaves and identification of their volatile compound compositions. Innovative Food Sci. and Emerging Tech. 10(2): 289–292.
📄Wendorff WL, Riha WR, Muehlenkamp E (1993). Growth of molds on cheese treated with heat or liquid smoke. J. Food Protect. 56(11): 963-966.
📄Wendorff WL, Wee C (1997). Effect of smoke and spice oils on growth of molds on oil-coated cheeses. J. Food Protect. 60(2): 153-156.
📄Yaseen T, D’Onghia AM (2012). Fusarium spp. associated to citrus dry root rot: an emerging issue for Mediterranean citriculture. Acta Hortic 940:647–655
📄Yazdanpanah L., Mohamadi N (2014). Antifungal activity of Satureja hortensis L. essential oil against Alternaria citri. European Journal of Experimental Biology, 4(1):399-403
📄Yeşil Çeliktaş, O., Hames Kocabaş, E.E., Bedir, E., Vardar Sukan, F., Özek, T., Baser, K.H.C., 2007. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry, 100: 553-559.
📄Yohalem D, Passey T (2011). Amendment of soils with fresh and post-extraction lavender (Lavandula angustifolia) and lavandin (lavandula×intermedia) reduce inoculum of verticillium dahliae and inhibit wilt in strawberry. Applied Soil Ecology, 49, 187–196.
