This study was carried out in order to determine the effect of different row spacing on yield and yield components of some safflower (Carthamus tinctorius L.) varieties in 2016-2017 vegetation period, winter seeding under the conditions of Kızıltepe plain in Mardin province. As used a material to four different safflower varieties (Asol, Olas, Linas and Balci) were used for different row spacing (10, 20, 30 and 40 cm). In all these applications, the distance intra row is kept constant to 10 cm. The research was established with three replications in according to Randomized Parcel Trial Design in Random Blocks. The varieties (Asol, Linas, Balci, Olas) are placed randomly on the main parcels, with the spacing between 10, 20, 30 and 40 cm. According to the results of the research; The highest yield was obtained from Linas with 206.33 kg da^-1 and the lowest yield was obtained from Olas with 184.42 kg da^-1. It has been determined that Linas varieties provide better adaptation to Kızıltepe ecological conditions. The highest yield was obtained from Linas varieties with 262,33 kg da^-1 at the 40 cm row spacing, and the lowest decare yield was obtained from Olas with 163,69 kg da^-1 at a spacing of 20 cm. While the lowest plant height was taken from Balcı variety with 164,67 cm and 10 cm row spacing, the highest plant height was obtained from Linas varieties with 183,00 cm and 10 cm row spacing. When looking at the average of varieties; There were determined between that the number of branches per plant 12.30-16.14 pieces (10-40 cm), the number of table per plant 12.58-16.49 pieces (10-40 cm), table diameter 2.29-2.40 mm (40-30 cm), seed yield per plant 9.20-15.86 g plant^-1 (10-40 cm), 1000 seed weight 32.57-35.04 g (30-40 cm), protein content %18,69-20,25 (10-40 cm), and the rate of oil 41.16-41.54% (30-10).