The Effect of Various Additives Addition to Barley-Vetch Mixtures on Silage Quality Characteristics and In vitro Digestibility

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Legumes, grains, in vitro, silage


In this study, it was aimed to determine the effects of adding different levels of molasses and fructose syrup to barley-vetch mixtures, one of the roughage sources produced as a second product in cotton fields in Şanlıurfa province, on some silage quality characteristics and ruminal methane gas formation by in vitro gas production technique. In the study, the additive-free silage group constituted the control group, while the silages, prepared by adding different levels of fructose syrup (0.1%, 0.2%, 0.3%, 0.4%) and molasses (0.5%, 1%, 1.5%, and 2%) to the control group, formed the treatment groups. In the study, while in vitro organic matter digestion (IVOMD) and metabolic energy (ME) values ​​of silages increased in all experimental groups compared to the control group, CH4 gas production decreased. The lowest pH value among the silage groups was obtained from the silage prepared with the addition of 1.5% and 2% molasses. It was observed that the ammonia nitrogen (NH3-N/TN) value of the silages decreased in the silage prepared by adding 1.5% molasses. While the highest amount of CO2 was determined in the control group, the lowest value was determined in the silage group with 1.5% molasses added. The highest value in terms of lactic acid content was determined in the control group, while the lowest was determined in the silage group with 0.5% molasses. When the acetic acid contents of the silages were examined, it was seen that all additives decreased the acetic acid contents of the silages compared to the control silage. As a result, it was concluded that the addition of 1.5% molasses or 0.4% fructose syrup additive had positive effects on silage fermentation, improved in vitro organic matter digestion and metabolic energy values, and could be used as it was economical and applicable.


Ali, N., Wang, S., Zhao, J., Dong, Z., Li, J., Nazar, M., Shao, T. 2020. Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota. Bioresource Technology, 314: 123606.

AOAC. 1990. Official methods of analysis of the association of official analytical chemists, Vol. II, 15th ed. Sec.985.29. The Association: Arlington, VA.

AOAC. 2005. Official methods of analysis. 18th ed. association of official analytical chemists. Arlington, VA, USA.

Arslan, C., Tufan, T., Avci, M., Kaplan, O., Uyarlar, C. 2020. Effects of molasses, barley, oak tannins extracts and previously fermented juice addition on silage characteristics, in vitro organic matter digestible and metabolisable energy content of grass silage. Fresenius Environmental Bulletin, 29(8): 6533-6542.

Ashbell, G., Weinberg, Z.G., Azrieli, A., Hen, Y., Horev, B. 1991. A simple system to study the aerobic determination of silages. Canadian Agricultural Engineering, 34: 171-175.

Avcı, M., Ayaşan, T. 2007. Preparation of silage with forage crops. Öztürk A. (Editor). Practical Cattle Breeding. Ankara, Ministry of Agriculture and Rural Affairs Non-formal Farmer Education Project, 205-222.

Bingöl, N.T., Baytok., E. 2003. The effects of some silage additives in sorghum silage on the silage quality and ruminal degradabilities of nutrients I. The effects on silage quality. Turkish Journal of Veterinary and Animal Sciences, 27(1): 15-20.

Bingöl, N.T., Bolat, D., Karslı, M.A., Akça, İ. 2009. Effects of molasses addition into barley-sainfoin mixture at varying levels on silage quality and digestibility. Veterinary Sciences and Practices, 4(1): 23-30.

Broderick, G.A., Mertens, D.R., Simons, R. 2002. Efficacy of carbohydrate sources for milk production by cows fed diets based on alfalfa silage. Journal of Dairy Science, 85(7): 1767-1776.

Canbolat, Ö., Kalkan, H., Filya, İ. 2013. The use of honey locust pods as a silage additive for alfalfa forage. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2): 291-297.

Carr, P.M., Horsley, R.D., Poland, W.W. 2004. Barley, oat, and cereal–pea mixtures as dryland forages in the northern Great Plains. Agronomy Journal, 96(3): 677-684.

Danner, H., Holzer, M., Mayrhuber, E., Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology, 69(1): 562-567.

De Man, J.C., Rogosa, D., Sharpe, M.E. 1960. A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology, 23(1): 130-135.

Demirel, R., Saruhan, V., Baran, M. S., Andiç, N., Demirel, D.Ş. 2010. Farklı karışım oranlardaki ak üçgül ve arpanın silolanma özelliklerinin tespit edilmesi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 20(1): 26-31.

Dubois, M., Giles, K.A., Hamilton, J.K., Rebes, P.A., Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356

Ergün, A., Tuncer, Ş.D., Çolpan, İ., Yalçın, S., Yıldız, G., Küçükersan, M.K., Küçükersan, S., Şehu, A., Saçaklı, P. 2013. Yemler, Yem hijyeni ve teknolojisi. Ankara University Faculty of Veterinary Medicine Extended 5th Edition. Ankara.

Görü, N., Seydoşoğlu, S. 2021. Determination of silage quality of some winter cereals (oat, barley, rye and triticale) mixed with common vetch (Vicia sativa L.). SDU Journal of the Faculty of Agriculture, 16(1): 26-33.

Haigh, P.M. 1990. Effect of herbage water‐soluble carbohydrate content and weather conditions at ensilage on the fermentation of grass silages made on commercial farms. Grass and Forage Science, 45(3): 263-271.

Kendall, N.V.G. 1978. Abnormal silages and silage related disease problems. Literature Review on Fermentation of Silage-A Review. Grants-In-Aid Committee. National Feed Ingredients Association. One Corporate Place, Suite, 360.

Li, M., Zi, X., Zhou, H., Hou, G., Cai, Y. 2014. Effects of sucrose, glucose, molasses and cellulase on fermentation quality and in vitro gas production of king grass silage. Animal Feed Science and Technology, 197: 206-212.

Menke, K.H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7-55.

Ozkan, U. 2020. Comparative overview and evaluation of Turkey’s forage crops agriculture. Turkish Journal of Agricultural Engineering Research, 1(1): 29-43.

Özyazici, M.A., Seydoşoğlu, S., Açikbaş, S. 2022. Determination of silage quality of fenugreek (Trigonella foenum-graecum L.) with oat (Avena sativa L.) and rye (Secale cereale L.) mixtures. Turkish Journal of Nature and Science, 11(3): 102-109.

Playne, M.J, McDonald, P. 1966. The buffering constituent of herbage and of silage, Journal of the Science of Food and Agriculture, 17, 264-268.

Polan, C.E., Stieve, D.E., Garrett, J.L. 1998. Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant. Journal of Dairy Science, 81(3): 765-776.

Seydoşoğlu, S. 2019. Investigation of the effect of fodder pea (Pisum sativum L.) and barley (Hordeum vulgare L.) herbages mixed at different rates on silage and feed quality. Journal of Agriculture Faculty of Ege University, 56(3): 297-302.

SPSS Inc. 1991. SPSS statistical algorithms. SPSS Incorporated.

Suzuki, M., Lund, C.W. 1980. Improved gas-liquid chromatography for simultaneous determination of volatile fatty acids and lactic acid in silage. Journal of Agricultural and Food Chemistry, 28(5): 1040-1041.

Taylor, C.C., Ranjit, N.J., Mills, J.A., Neylon, J.M., Kung Jr, L. 2002. The effect of treating whole-plant barley with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for dairy cows. Journal of Dairy Science, 85(7): 1793-1800.

Turan, N. 2019. Determination of chemical composition and quality properties of silage produced by mixing green Hungarian vetch and barley in various proportions. European Journal of Science and Technology, 17: 787-793.

Turan, N., Seydoşoğlu, S. 2020. Evaluation the effect of pure and various proportions of alfalfa, sainfoin and italian ryegrass on silage and feed quality. Turkish Journal of Agricultural and Natural Sciences, 7(3): 526–532.

Van Soest, P.V., Robertson, J.B., & Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10): 3583-3597.

Yolcu, H., Tan, M. 2008. General view to Turkey forage crops cultivation. Journal of Agricultural Sciences, 14(3): 303-312.

Zhang, Y.G., Xin, H.S., Hua, J.L.2010. Effects of treating whole-plant or chopped rice straw silage with different levels of lactic acid bacteria on silage fermentation and nutritive value for lactating Holsteins. Asian-Australasian Journal of Animal Sciences, 23(12): 1601-1607.




How to Cite

AYDIN, S. S. . (2022). The Effect of Various Additives Addition to Barley-Vetch Mixtures on Silage Quality Characteristics and In vitro Digestibility. ISPEC Journal of Agricultural Sciences, 6(4), 743–751.