Molecular Investigation of Equine Infectious Anemia Virus in Horses in Kars Province


Abstract views: 269 / PDF downloads: 227

Authors

DOI:

https://doi.org/10.5281/zenodo.8020301

Keywords:

Horse, EIAV, blood, Kars, nested RT-PCR

Abstract

The aim of this study was to determine the prevalence of Equine Infectious Anemia virus in horses in Kars by polymerase chain reaction (PCR) method. Horses; they have always had an important place in human life since the first day they are domesticated. Equine Infectious Anemia (EIA), which causes heavy losses in the horse population, is listed as one of the equine diseases that must be reported by the World Organization for Animal Health (OIE). Equine Infectious Anemia Virus (EIAV) is considered a barrier to the equine industry as it causes a persistent infection in horses. In this study, blood samples were taken from 120 horses in small family businesses in Kars region in accordance. Following RNA extraction from blood samples, nested RT-PCR analyzes were performed with specific primers in EIAV. All of the horses examined were negative for Equine Infectious Anemia Virus. Infectious Anemia of Horses, which is among the diseases that should be reported in our country, was observed to be absent in horses in Kars. It is recommended that the horse population should be regularly monitored throughout the country in order to ensure the country's permanent free status from Equine Infectious Anemia Virus.

References

Acar, D.B., Gür, S., Gürçay M., Özenç E.A., 2016. Serologic investigation for Equine Viral Arteritis and Equine Infectious Anemia Virus infections in horses in Afyonkarahisar, Ankara and Eskişehir provinces. Turkey Kocatepe Veterinary Journal, 9(3): 159-164.

Akçay, S., 1953. Yurdumuz atlarında anemi enfeksiyozun zuhuru ve anatomo-histopatolojik teşhisinin değeri. Türk Veteriner Hekimler Derneği Dergisi, 76-77: 479-505.

Albayrak, H., Özan, E., 2010a. Serosurveillance for equine infectious anemia in the Ardahan province of Turkey. Tropical Animal Health and Production, 42(8): 1593-1595.

Albayrak, H., Özan, E., 2010b. Orta Karadeniz bölgesinde ruminant ve tek tırnaklılarda kan emici sineklerle nakledilen bazı arboviral enfeksiyonların seroprevalansı. Kafkas Universitesi Veterinerlik Fakültesi Dergisi, 16(1): 33-36.

Alnaeem, A.A., Hemida, M.G., 2019. Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014–2016. Veterinary World, 12(5): 719–723.

APHIS, 2006. Equine infectious anemia: uniform methods and rules effective. The United States Department of Agriculture Publications, USA.

Ataseven, V.S., Arslan, H.H., 2005. Equine infectious anemia in mules, donkeys, and horses: Epidemiologic studies in the different geographic regions of Turkey. Journal of Equine Veterinary Science, 25: 439-441.

Burgu, İ, Akça, Y., Toker, A., Alkan, F., 1989. Atlarda enfeksiyöz aneminin serolojik olarak araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 36(1): 123-128.

Cappelli, K., Capomaccio, S., Cook, F.R., Felicetti, M., Marenzoni, M.L., Coppola, G., Passamonti, F., 2011. Molecular detection, epidemiology, and genetic characterization of novel European field isolates of equine infectious anemia virus. Journal of Clinical Microbiology, 49(1): 27-33.

Cheevers, W.P., McGuire, T.C., 1985. Equine infectious anemia virus: immunopathogenesis and persistence. Reviews of Infectious Diseases, 7(1):83-88.

Cook, R.F., Issel, C.J., Montelaro, R.C., 1996. Equine infectious anemia. In Virus infections of equines, Vol. 6 of Virus infections of vertebrates. Elsevier Science BV, the Netherlands, 297–323.

Cruz, F., Fores, P., Ireland, J., Moreno, M.A., Newton R., 2015. Freedom from equine infectious anemia virus infection in Spanish purebred horses. Veterinary Record Open, 2(1): e000074.

González Del Pino, F.J., 2011. Anemia infecciosa equina (AIE): presentación de un cuadro clínico (Equine infectious anemia (AIE): a case report). Revista Electronica de Veterinaria, 12(10): 1–12.

Del Piero, F., Wilkins, P.A, Lopez J.W., Glaser, A.L., Dubovi, E.J, Schlafer, D.H., Lein D.H., 1997. Equine arteritis virus in newborn foals: clinical, pathological, serological, microbiological and immunohistochemical observations. Equine Veterinary Journal, 29(3): 178-185.

DEFRA, 2006. Equine infectious anemia: potential risk factors for the introduction of the virus the Greate Britain from EU member states and countries neighbouring the EU. International Disease Monitoring Units Qualitative Risk Assessment, 1:1-16.

Gaudaire, D., Lecouturier, F., Ponçon, N., Morilland, E., Laugier, C., Zientara, S., Hans, A., 2018. Molecular characterization of equine infectious anaemia virus from a major outbreak in southeastern France. Transboundary And Emerging Diseases, 65(1): e7-e13.

Hakioğlu, F., 1955. Yurdumuz atlarında anemia infectiose hastalığının teşhisi için karaciğerde histopaolojik araştırmalar. Ankara Üniversitesi Veteriner Fakültesi Yayınları, No:61. A Ü Basımevi, Ankara.

Hammond, S.A., Li, F., McKeon Sr, B.M., Cook, S.J., Issel, C.J., Montelaro, R.C., 2000. Immune responses and viral replication in long-term inapparent carrier ponies inoculated with equine infectious anemia virus. Journal Of Virology, 74(13): 5968-5981.

Issel, C.J., Foil, L.D., 2015. Equine infectious anaemia and mechanical transmission: man and the wee beasties. Revue scientifique et technique - Office international des épizooties, 34(2): 513–523.

Kırmızıgül, A.H., Yıldırım, Y., Gökçe, E., Ataseven, V.S., 2009. Serologic evaluation of the equine infectious anemia in Kars and ArdahanTurkey. Kafkas Universitesi Veteriner Fakültesi Dergisi, 15(1): 77-80.

OIE, 2019. Manual od Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds, and Bees). 5th ed. Office International Des Epizootics, Paria, France.

Lignee, M., 1843. Memoire et observations sur une maladie de sang, connue sous le nom d’anhemie hydrohemie cachexie aqueuse du cheval. Rec Med Vet Ec Alfort Journal, 20: 30-45.

Marenzoni, M.L., Cuteri, V., Parri, F.D., Danzetta, M.L., Yilmaz, Z., Yaramis, C. P., Costarelli, S., 2013. A pilot study on the epidemiological status of equine infectious anaemia, equine viral arteritis, glanders, and dourine in Turkey. Turkish Journal of Veterinary & Animal Sciences, 37(1): 76-80.

Noyan, A., 1958. Anemia infectiosa equorumlu safkan arap atlarında hematolojik araştırma. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 5: 209-241.

Romo-Sáenz, C.I., Tamez-Guerra, P., Olivas-Holguin, A., Ramos-Zayas, Y., Obregón-Macías, N., González-Ochoa, G., Gomez-Flores, R., 2021. Molecular detection of equine infectious anemia virus in clinically normal, seronegative horses in an endemic area of Mexico. Journal of Veterinary Diagnostic Investigation, 33(4): 758-761.

Sellon, D.C., Fuller, F.J., McGuire, T.C., 1994. The immunopathogenesis of equine infectious anemia virus. Virus Research, 32(2): 111–138.

Tarım ve Köyişleri Bakanlığı: İhbari Mecburi Hayvan Hastalıkları ve Bildirimine İlişkin Yönetmelik. 22 Ocak 2011 tarih ve 27823 sayılı Resmi Gazete. Erişim Adresi: https://www.resmigazete .gov.tr/eskiler/2011/01/20110122-4.htm

Tigre, D.M., Brandão, C.F.L., de Paula, F. L., Chinalia, F.A., Campos, G.S., Sardi, S.I., 2017. Characterization of isolates of equine infectious anemia virus in Brazil. Archives of Virology, 162: 873-877.

Turan, N., Yılmaz, H., Uysal, A., Arslan, M., 2002. Seronegative findings on the investigations of equine infectious Anemia in the Marmara Region of Turkey. Turkish Journal of Veterinary & Animal Sciences, 26(2): 299-302.

TÜİK, 2022. Erişim Tarihi: 01.08.2022 Erişim adres,: https://data.tuik.gov.tr/Bu lten/Index?p=Hayvansal-Uretim-Istatisti kleri-Aralik-2021-45593.

Yapkıç, O., Yavru, S., Kale, M., Bulut, O., Şimşek, A., Şahna, K.C., 2007. An investigation of equine infectious anemia infection in the Central Anatolia region of Turkey. Journal of the South African Veterinary Association, 78(1): 12-14.

Vallée, H., Carré, H., 1904. Sur la natur infectieuse de L'anémie du cheval. Comptes Rendus de l'Académie des Sciences, 139: 331-333.

Published

2023-06-21

How to Cite

KOÇ AKPINAR, R., ÇELİK, S. ., AKMAN, A. ., & KIZILTEPE, Şemistan . (2023). Molecular Investigation of Equine Infectious Anemia Virus in Horses in Kars Province. ISPEC Journal of Agricultural Sciences, 7(2), 263–270. https://doi.org/10.5281/zenodo.8020301

Issue

Section

Articles