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Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, Çanakkale
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Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, Çanakkale
Abstract
There is an urgent need to evaluate and optimize use of different sized pollen grains of inducer lines to increase haploid seed production in maize breeding programs. Therefore, this study aimed to evaluate the effect of pollen size on haploid seed production in maize under greenhouse conditions to characterize pollen samples taken from an inducer maize line by morphological, molecular and spectrophotometric measurements. Three donors and one inducer line were used as the experimental material. The pollen samples of inducer line were collected and filtered into two different sizes (50µ and 100µ) using a special vacuum filtration device. Morphological measurements based on image analysis were made, spectral data were collected in Near Infrared Reflectance (NIR) and Raman spectroscopy devices. The protein bands were examined with SDS-PAGE analysis on the collected pollen and seed samples. Haploid induction rates of the seed samples obtained after pollination of donor materials with sized-pollen samples were determined. The results of the study indicated spectral and molecular differences in the pollen samples separated by 50 and 100µ filters. Haploid induction rate (HIR) values ranged 7.40% -18.9% in the used donors, however higher haploid seed formation was observed in small-sized pollen samples compared to the others.
Keywords
Pollen,ploidy,protein,navajo,Zea mays L.
How to Cite
PINAR, G. ., & KAHRIMAN, F. (2023). Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize. ISPEC Journal of Agricultural Sciences, 7(2), 423–434. https://doi.org/10.5281/zenodo.8063363
📄Begcy, K., Dresselhaus, T., 2017. Tracking maize pollen development by the Leaf Collar Method. Plant Reproduction, 30(4): 171–178.
📄Bell, K.L., De Vere, N., Keller, A., Richardson, R.T., Gous, A., Burgess, K.S., Brosi, B.J., 2016. Pollen DNA barcoding: current applications and future prospects. Genome, 59(9): 629-40.
📄Birchler, J.A., Newton, K.J., 1981. Modulation of protein levels in chromosomal dosage series of maize: the biochemical basis of aneuploid syndromes. Genetics, 99: 247–266.
📄Bujang, J.S., Zakaria, M.H., Ramaiya, S.D., 2021. Chemical constituents and phytochemical properties of floral maize pollen. PLoS One, 16(2): e0247327.
📄Chaikam, V., Molenaar, W., Melchinger, A.E., Boddupalli, P.M., 2019. Doubled haploid technology for line development in maize: technical advances and prospects. Theoretical and Applied Genetics, 132: 3227-3243.
📄Chaikam, V., Prasanna, B.M., Mahuku, G., 2012. Doubled haploid technology in maize breeding: Theory and practice. In: B.M. Prasanna, V. Chaikam, G. Mahuku (Eds), Maternal Haploid Detection Using Anthocyanin Markers, Mexico, pp. 21-24.
📄Chase, S.S., 1969. Monoploids and monoploid-derivatives of maize (Zea maysL.). The Botanical Review, 35: 117–167.
📄Chidzanga, C., Muzawazi, F., Midzi, J., Hove, T., 2017. Production and use of haploids and doubled haploid in maize breeding: A review. African Journal of Plant Breeding, 4: 201-213.
📄Fonseca, A.E., Westgate, M.E., Doyle, R.T., 2002. Application of fluorescence microscopy and image analysis for quantifying dynamics of maize pollen shed. Crop Science, 42(6): 2201-6.
📄Geiger, H.H., 2009. Doubled haploids. In: J.L. Bennetzen, S. Hake (Eds.), Maize handbook -volume II: genetics and genomics. Springer Science and Business Media, New York, pp. 641- 657.
📄Gilles, L.M., Martinant, J.P., Rogowsky, P.M., Widiez, T., 2017. Haploid induction in plants. Current Biology, 27: 1095-1097.
📄Iqbal, J., Shinwari, Z.K., Rabbani, M.A., Khan, S.A., 2014. Genetic variability assessment of maize (Zea mays L.) germplasm based on total seed storage proteins banding pattern using SDS-PAGE. European Academic Research, 2(2): 2144–60.
📄Jacquier, N.M., Gilles, L.M., Martinant, J.P., Rogowsky, P.M., Widiez, T., 2021. Doubled haploid technology. In: J.M. Segui-Simarro (Ed), Maize in Plant a Haploid Inducer Lines: a Cornerstone for Doubled Haploid Technology, Mexico, pp. 25 – 48.
📄Joujeh, R., Zaid, S., Mona, S., 2019. Pollen morphology of some selected species of the genus Centaurea L. (Asteraceae) from Syria. South African Journal of Botany, 125: 196-201.
📄Kendel, A., Zimmermann, B., 2020. Chemical analysis of pollen by FT-Raman and FTIR spectroscopies. Frontiers in Plant Science, 11: 352.
📄Li, X., Meng, D., Chen, S., Luo, H., Zhang, Q., Jin, W., Yan, J., 2017. Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction. Nature Communications, 8(1): 1-9.
📄Liu, Q., Yang, J., Wang, X., Zhao, Y., 2023. Studies on pollen morphology, pollen vitality and preservation methods of gleditsia sinensis Lam. (Fabaceae). Forests, 14(2): 243.
📄Mitiku, T., 2022. Review on haploid and double haploid Maize (Zea mays) breeding technology. International Journal of Agricultural Science and Food Technology, 8(1): 52-58.
📄Mondol, A.S., Patel, M.D., Rüger, J., Stiebing, C., Kleiber, A., Henkel, T., Popp, J., Schie, I.W., 2019. Application of high-throughput screening Raman spectroscopy (HTS-RS) for label-free identification and molecular characterization of pollen. Sensors, 19(20): 4428.
📄Patil, I., 2021. Visualizations with statistical details: The 'ggstatsplot' approach. Journal of Open Source Software, 6(61): 3167.
📄Pereira, S.G., Guedes, A., Abreu, I., Ribeiro, H., 2021. Testing the Raman parameters of pollen spectra in automatic identification. Aerobiologia, 37: 15-28.
📄Pospiech, M., Javůrková, Z., Hrabec, P., Štarha, P., Ljasovská, S., Bednář, J., Tremlová, B.,2021. Identification of pollen taxa by different microscopy techniques. PloS One, 16(9): e0256808.
📄Qiu, F, Liang, Y, Li, Y, Liu, Y, Wang, L, Zheng, Y., 2014. Morphological, cellular and molecular evidences of chromosome random elimination in vivo upon haploid induction in maize. Current Plant Biology, 1: 83-90.
📄Radev, Z., 2018. Variety in protein content of pollen from 50 plants from Bulgaria. Bee World, 95(3): 81-83.
📄Schneider, C.A., Rasband, W.S., Eliceiri, K.W., 2012. NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7): 671-675.
📄Soares, T.L., Jesus, O.N., Souza, E.H., Rossi, M.L., Oliveira, E.J., 2017. Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba. Anais da Academia Brasileira de Ciências, 90: 2381-2396.
📄Swapna, M., Sarkar, K.R., 2011. Anomalous fertilization in haploid inducer lines in maize (Zea mays L). Maydica, 56(3).
📄Tuna, G.S., Uyanik, B., Özdemir, E.E., Dalgiç, G., Mengi, Y., Korkut K.Z., 2019. Electrophoretic characterization of inbred maize lines. International Advanced Researches and Engineering Journal, 3(2): 86-92.
📄Ünlü, E., Mutlu, E., Polat, M., Çeri, S., Kahrıman, F., 2018. Diversity among Turkish maize landraces based on protein band analyses and kernel biochemical properties. Journal of Crop Improvement, 32(2): 175-187.
📄Vanous, K., Vanous, A., Frei, U.K. Lübberstedt, T., 2017. Generation of maize (Zea mays) doubled haploids via traditional methods. Current Protocols in Plant Biology, 2(2): 147-157.
📄Wang, Y., Lv, Y., Liu, H., Wei, Y., Zhang, J., An, D., Wu, J., 2018. Identification of maize haploid kernels based on hyperspectral imaging technology. Computers and Electronics in Agriculture, 153: 188-195.
📄Williams, P.J., Kucheryavskiy, S., 2016. Classification of maize kernels using NIR hyperspectral imaging. Food Chemistry, 209: 131-138.
📄Wizenberg, S.B., Weis, A.E., Campbell, L.G., 2020. Comparing methods for controlled capture and quantification of pollen in Cannabis sativa. Applications in Plant Sciences, 8(9): e11389.
📄Yu, J., Roy, S.K., Kamal, A.H.M., Cho, K., Kwon, S-J., Cho, S-W., So, Y-S., Holland, Jb., Woo, Sh., 2014. Protein profiling reveals novel proteins in pollen and pistil of W22 (ga1; Ga1) in Maize. Proteomes, 2: 258-271.
📄Zhou, L.Z., Juranić, M., Dresselhaus, T., 2017. Germline development and fertilization mechanisms in maize. Molecular Plant, 10(3): 389-401.
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Fatih KAHRIMAN
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