HaeIII Polymorphism of Growth Hormone (GH-1) Gene in Some Goat Breeds Reared in Turkey by Using PCR-RFLP Method

Abstract views: 166 / PDF downloads: 110





Rapeseed, variety, organic fertilizers, yield, yield components



The purpose of this study is to determine HaeIII polymorphism in the exon 2 and 3 regions of the growth hormone -1 (GH-1) gene that regulates tissue growth and muscle development in six different goat breeds reared in Turkey. The HaeIII polymorphism in GH-1 gene (422 bp) was investigated by Restriction Fragment Length Polymorphism (PCR-RFLP) methodology in 303 goats including 52 Hair, 51 Angora, 50 Honamlı, 50 Halep, 50 Saanen and 50 heads of Kilis breeds. Two alleles (A and B) and 3 genotypes (AA, BB and AB) were identified in the study. A and B allele frequencies in Hair, Angora, Honamlı, Halep, Saanen and Kilis breeds were found to be 0.4038 and 0.5962, 0.4314 and 0.5686, 0.4600 and 0.5400, 0.4500 and 0.5500, 0.3800 and 0.6200, 0.5400 and 0.4600, respectively. AA, AB and BB genotype frequencies were found to be 00.019, 0.769 and 0.212 (P<0.05), 0.235, 0.392 and 0.373 (P>0.05), 0.080, 0.760 and 0.160 (P<0.05), 0.060, 0.780 and 0.160 (P<0.05), 0.160, 0.440 and 0.400 (P>0.05), 0.160, 0.760 and 0.080 (P<0.05), respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4439 and 0.5561, and AA, AB and BB genotype frequencies were found to be 0.119, 0.650 and 0.231 (P>0.05). As a result, it was determined that goat population had high heterozygosity (0.494) in general and was not in Hardy-Weinberg equilibrium in terms of HaeIII polymorphism in the exon 2 and 3 regions of the GH-1 gene (P>0.05).


Anonim‎, ‎2018‎. (http://www.tuik.gov.tr/Start.do), (Access date: 03.05.2018).‎

Anonymous, ‎‎2017‎, Capra Hircus Breed San Clemente Chromosome 5, ASM170441v1, Whole Genome Shotgun Sequence.

(https://www.ncbi.nlm.nih.gov/nuccore/NC_030812.1?report=genbank&from=64862983‎ &to=64943172 &strand=true), (Access date: 05.07.2017)‎.

Bayan, J., Kharadi, V., Ramani, U., Janmeda, M., Tyagi, K., Dangar, N., Pandya, G., 2018. Genetic polymorphism of growth hormone gene exon-4 in surti and mehsani goats by PCR-RFLP. The Indian Journal of Veterinary Sciences And Biotechnolog, 14(1): 28-33.

Dettori, M.L., Rocchigiani, A.M., Luridiana, S., Mura, M.C., Carcangiu, V., Pazzola, M., Vacca, G.M., 2013. Growth hormone gene variability and its effects on milk traits in primiparous Sarda goats. Journal of Dairy Research, 80(3): 255-262.

Ilham, F., Rachman, S.D.A.B., Dagong, M.I.A., 2016. Genetic polymorphisms of growth hormone (GH) gene in Kacang goat population based on polymerase chain reactionrestriction fragment length polymorphism (PCRRFLP) identification. Livestock Research for Rural Development, 28: 9.

FAO, 2017. Food and Agriculture Data, (http://www.fao.org/statistics/en/), (Access Date: 19.06.2019).

Fries, R., 1993. Mapping the bovine genome: methodological aspects and strategy. Animal Genetics, 24: 111–116.

Ge, W., Davis, M., Hines, H., Irvin, K., Simmen, R., 2003. Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle, Journal of Animal Science, 81(3): 641-648.

Hua, G,, Chen, S,, Yu, J,, Cai, K,, Wu, C,, Li, Q,, Zhang, C,, Liang, A,, Han, L., Geng, L., 2009. Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks, Meat Science, 81(2): 391-395.

Mahrous, K.F., Abdel-Aziem, S.H., Abdel-Hafez, M.A., Abdel-Mordy, M., Rushdi, H.E., 2018. Polymorphism of growth hormone gene in goat breeds in Egypt, Bulletin of the National Research Centre, 42(1): 35.

Mete, Ö.S., 2016. Growth hormone gene polymorphism and its relationship with growth characteristics in domestic hair goats. Master Thesis, T.C. Adnan Menderes University, İzmir.

Kaymakcı, M., Koşum, N., Taşkın, T., Akbaş, Y.‎, ‎2006. A research on determination of some yield characteristics in Menemen sheep‎. Ege University Faculty of Agriculture Journal, 43(1): 63-74.‎

Kaymakcı, M.A.Y.‎, ‎‎1997. Goat Breeding, Ege University Faculty of Agriculture, No:294, İzmir.

Kunda, R.M., Volkandari, S.D., Rumanta, M., Kakisina, P., 2020. Polymorphism of growth hormone (GH) gene in lakor goat from lakor island of southwest maluku regency. Buletin Peternakan, 44(4).

Shareef, M., Basheer, A., Zahoor, I., Anjum, A.A., 2018. Polymorphisms in growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene and ‎their association with growth traits in beetal goat. Pakistan Journal of Agricultural Sciences, 55(3): 713-720.

Othman, O.E., Alam, S.S., El-Kader, H.A.A., Abd-El-Moneim, O.M., 2015. Genotyping of growth hormone gene in Egyptian small ruminant breeds. Biotechnology, 14(3): 136.

Alakilli, S.Y., Mahrous, K.F., Salem, L.M., Ahmed, E.S., 2012. Genetic polymorphism of five genes associated with growth traits in goat. African Journal of Biotechnology, 11(82): 14738-14748.

Singh, P.P., Tomar, S.S., Thakur, M.S., Kumar, A., 2015. Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat. Veterinary World, 8(3): 382.

Wallis, M., Lioupis, A., Wallis, O. C., 1998. Duplicate growth hormone genes in sheep and goat. Journal of Molecular Endocrinology, 21(1): 1-5.

Wickramaratne, S., Ulmak, B., Dixit, S., Kumar, S., Vyas, M., 2009. Use of growth hormone gene plymorphism in selecting osmanabadi and sangamneri goats. Tropical Agricultural Research 21(4): 398-411.

Yao, J., Aggrey, S.E., Zadworny, D., Hayes, J.F., Kühnlein, U., 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphism (SSCP) analysis and their association with milk production traits in Holsteins. Genetics, 144(4): 1809-1816.

Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H., Mao, J.X., Yang, R.C., Ye, Z.H., 1997. POPGENE, the user-friendly shareware for population genetic analysis. Molecular biology and biotechnology centre, University of Alberta, Canada.

Zhang, C., Liu, Y., Huang, K., Zeng, W., Xu, D., Wen, Q., Yang, L., 2011, The association of single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds, Genetics and Molecular Biology, 34 (1): 49-55.




How to Cite

AMINE OUCHAR, M. A., DAĞ, B. ., & AYTEKİN, İbrahim . (2023). HaeIII Polymorphism of Growth Hormone (GH-1) Gene in Some Goat Breeds Reared in Turkey by Using PCR-RFLP Method. ISPEC Journal of Agricultural Sciences, 7(3), 693–700. https://doi.org/10.5281/zenodo.8373425