Melatonin Receptor 1A (MTNR1A) Gene Polymorphism in Cross-Bred Hamdani Sheep: A Preliminary Study
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https://doi.org/10.5281/zenodo.10815493Keywords:
Cross-bred Hamdani sheep, MTNR1A, polymorphism, PCR-RFLP, reproductionAbstract
Melatonin plays a key role in regulating several vital physiological processes, including the maturation and functioning of the reproductive system, pubertal development, seasonal reproduction, and adaptation. The activation of the melatonin hormone is facilitated by melatonin receptors. This study aimed to investigate melatonin receptor 1A (MTNR1A) gene polymorphism in cross-bred Hamdani sheep. A total of 102 cross-bred Hamdani ewes were used as animal material. DNA was extracted from whole blood. The 824 bp PCR products from the exon II region of the MTNR1A gene underwent analysis for restriction fragment length polymorphism (RFLP) using the RsaI restriction enzyme. In the study, RR, Rr, and rr genotypes were detected for MTNR1A gene in cross-bred Hamdani sheep. Genotype frequencies for RR, Rr, rr were 0.69, 0.27, and 0.04, respectively. Allele frequencies were detected as 0.82 and 0.18 for R and r alleles, respectively. In conclusion, more comprehensive research investigating association between MTNR1A genotypes and reproductive traits should be carried out in cross-bred Hamdani sheep.
References
Antonopoulou, D., Giantsis, I.A., Symeon, G. K., Avdi, M., 2023. Association of MTNR1A and GDF9 gene allelles with the reproductive performance, response to oestrus induction treatments and prolificacy, in improved and non‐improved local indigenous sheep breeds. Reproduction in Domestic Animals, 58(11): 1532-1541.
Arjoune, A., Alsaleh, A.B., Messaoudi, S.A., Chelbi, H., Jelassi, R., Assidi, M., Najar, T., Haddad, B., Sirard, M.A., 2023. Analysis of MTNR1A genetic polymorphisms and their association with the reproductive performance parameters in two Mediterranean sheep breeds. Animals, 13(3): 448,1-13.
Avanus, K., Altinel, A., 2016. Identification of genetic variation of melatonin receptor 1a (mtnr1a) gene in kıvırcık breed ewes by mnli and rsai restriction enzymes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(4): 571-576.
Bakır, G., Mikail, N., 2019. Siirt ilindeki küçükbaş hayvancılık işletmelerinin yapısal durumu. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 50(1): 66-74.
Barığ, M., Çelikyürek, H., 2023. Van ili koyunculuk işletmelerinde kullanılan teknolojilerin tespiti. Journal of Anatolian Environmental and Animal Sciences, 8(4):576-589.
Chemineau, P., Bodin, L., Migaud, M., Thiery, J. C., Malpaux, B., 2010. Neuroendocrine and genetic control of seasonal reproduction in sheep and goats. Reproduction in Domestic Animals = Zuchthygiene, 45(Suppl 3): 42–49.
Cosso, G., Nehme, M., Luridiana, S., 2021. Detection of polymorphisms in the mtnr1a gene and their association with reproductive performance in awassi ewes. Animals. 11(2): 1-11.
Doğan, H., Kutlu, M., 2022. Üreme Sezonu Dışında Kısa Süreli Progestagen ve PMSG ile senkronize edilen koyunlarda immunmodülatör kullanımının vaginal akıntı skoru ve konsepsiyon oranı üzerine etkisi. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 36(3): 196-199.
Elmacı, C., Sahin, S., Oner, Y., 2013. Distribution of different alleles of aromatase cytochrome P450 (CYP19) and melatonin receptor 1A (MTRN1A) genes among native Turkish sheep breeds. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(6): 929-933.
Fathy, H.A., Gouda, E.M., Gafer, J.A., Galal, M.K., Nowier, A.M., 2018. Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds. Archives Animal Breeding, 61(4): 505-516.
Fındık, M., 2017. Küçük ruminantlarda reprodüktif sürü yönetimi. Türkiye Klinikleri Journal Veterinary Science Obstetion Gynecol-Special Topics, 3: 96-98.
Kan, M., Gülçubuk, B., Kan, A., Küçükçongar, M., 2010. Coğrafi işaret olarak karaman divle tulum peyniri. Karamanoğlu Mehmetbey Üniversitesi Sosyal ve Ekonomik Araştırmalar Dergisi, 2010(2): 15-23.
Karadaş, K., 2018. Koyunculuk işletmelerinin sosyo-ekonomik durumu Hakkâri ili örneği. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 49(1): 29-35.
Luridiana, S., Cosso, G., Pulinas, L., Di Stefano, M.V., Curone, G., Carcangiu, V., Mura, M.C., 2020. New polymorphisms at MTNR1A gene and their association with reproductive resumption in sarda breed sheep. Theriogenology, 158: 438-444.
Luridiana, S., Mura, M.C., Daga, C., Cosso, G., Bodano, S., Farci, F., Zidda, F., Carcangiu, V., 2016. Influences of melatonin treatment, melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) gene polymorphisms on first conception in Sarda ewe lambs. Reproduction, Fertility and Development, 28(6): 750-756.
Martínez-Royo, A., Lahoz, B., Alabart, J.L., Folch, J., Calvo, J.H., 2012. Characterisation of the melatonin receptor 1A (MTNR1A) gene in the Rasa Aragonesa sheep breed: association with reproductive seasonality. Animal Reproduction Science, 133(3-4): 169-175.
Messer, L.A., Wang, L., Tuggle, C.K., Yerle, M., Chardon, P., Pomp, D., Womack, J.E., Barendse, W., Crawford, A.M., Notter, D.R., Rothschild, M.F., 1997. Mapping of the melatonin receptor la (MTNR1A) gene in pigs, sheep, and cattle. Mammalian Genome, 8: 368-370.
Mura, M.C., Cosso, G., Pulinas, L., Carcangiu, V., Luridiana, S., 2022. Reproductive resumption in winter and spring related to MTNR1A gene polymorphisms in sarda sheep. Animals, 12(21): 2947.
Pulinas, L., Starič, J., Cosso, G., Curone, G., Mura, M.C., Carcangiu, V., Luridiana, S., 2022. MTNR1A gene polymorphisms and reproductive recovery after seasonal anoestrus in different Mediterranean sheep breeds. Animal Reproduction Science, 236: 106905.
Saxena, V.K., B.K., Meena, A.S., Naqvi, S.M.K., 2015. Characterization of MTNR1A gene in terms of genetic variability in a panel of subtemperate and subtropical Indian sheep breeds. Journal of Genetics, 94: 715-721.
Şeker, I., Ozmen, O., Ertugrul, O., 2011. Polymorphism in melatonin receptor 1A (MTRN1A) gene in Chios, White Karaman and Awassi sheep breeds. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(5): 865-868.
Starič, J., Farci, F., Luridiana, S., Mura, M. C., Pulinas, L., Cosso, G., Carcangiu, V., 2020. Reproductive performance in three Slovenian sheep breeds with different alleles for the MTNR1A gene. Animal Reproduction Science, 216: 106352.
Tamer, B., Sariözkan, S., 2017. Yozgat merkez ilçede koyunculuk yapan işletmelerin sosyo-ekonomik yapısı ve üretim maliyetleri. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 14(1): 39-47.
TUIK, 2023. Hayvansal Üretim İstatistikleri. (https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-2023-49681), (Erişim Tarihi: 08.06.2023)
Turgut, A.O., Gülendağ, E., Koca, D., Üner, S., 2023. Milk composition traits of hamdani crossbreed sheep raised under extensive management. ISPEC Journal of Agricultural Sciences, 7(2): 271-279.
Turgut, A.O., Koca, D., 2024. Serum anti-müllerian hormone levels during estrus and diestrus phases of the estrous cycle and its possible effect on fertility in cross-bred hamdani sheep. Pakistan Veterinary Journal.
Varanlou, D., Hassani, S., Ahani Azari, M., Samadi, F., Zakizadeh, S., Khan Ahmadi, A.R., 2017. Association between MTNR1A and CYP19 genes polymorphisms and economic traits in kurdi sheep. Iranian Journal of Applied Animal Science, 7(1): 69-74.
Vibha, C.R., Ramakrishnappa, N., Naveen, G.S., Jayashree, R., Ravindranathe, B.M., 2018. MTNR-1A gene polymorphism in two tropical sheep breeds of South India. Indian Journal of Veterinary Sciences & Biotechnology, 14(2): 44-48.
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