Chemical Composition and Antioxidant Potential of Aristolochia maurorum Above-groud Extract: Phenolic and Phytochemical Analysis

Emine İncilay TORUNOGLU; Erdi Can AYTAR; Alper  DURMAZ; Betül AYDIN; Ayşe Melda ÇOLAK

doi:10.5281/zenodo.12787050

Authors

Emine İncilay TORUNOGLU Necmettin Erbakan University, Faculty of Medicine, Department of Medical Biochemistry, Konya https://orcid.org/0000-0003-4641-0067
Erdi Can AYTAR Usak University, Faculty of Agriculture, Department of Horticulture, Uşak https://orcid.org/0000-0001-6045-0183
Alper DURMAZ Artvin Coruh University, Ali Nihat Gok Yigit Botanical Garden Application and Research Center, Artvin https://orcid.org/0000-0001-6927-3283
Betül AYDIN Gazi University, Faculty of Science, Department of Biology, Ankara https://orcid.org/0000-0002-9092-1350
Ayşe Melda ÇOLAK Usak University, Faculty of Agriculture, Department of Horticulture, Uşak https://orcid.org/0000-0003-4475-275X

DOI:

https://doi.org/10.5281/zenodo.12787050

Keywords:

GC-MS analysis, antioxidant activity, Aristolochia maurorum, phytochemicals

Abstract

The aim of this study is to evaluate the antioxidant activity, chemical composition, phenolic components, and fatty acid content of extracts obtained from the aerial parts of Aristolochia maurorum. The above-groud of A. maurorum were collected from remote areas in Havza and Kavak districts of Samsun province, Turkey. The plants were dried, powdered, and extracted with methanol. The total phenolic contents, total flavonoid contents, total tannin contents, and total proanthocyanidin contents of the extracts were determined using spectrophotometric methods. Additionally, DPPH free radical scavenging activity was measured. The fatty acid contents were analyzed using gas chromatography-mass spectrometry (GC-MS). The methanol extract of A. maurorum above-groud contains high levels of phenolic compounds and exhibits strong antioxidant activity. The total phenolic content of the extract was found to be 12.24 ± 0.91 mg GAE/g, total flavonoid content 27.45 ± 0.31 mg QE/g, total tannin content 28.41 ± 0.31 mg GAE/g, and total proanthocyanidin content 155.24 ± 32.20 mg CAE/g. The DPPH free radical scavenging activity showed an IC₅₀ value of 506.29 ± 38.35 mg/mL. GC-MS analyses demonstrated that the extract contains various bioactive compounds, notably eicosane, which is reported to have anti-inflammatory, analgesic, and antipyretic properties. In conclusion, extracts derived from the above-groud of A. maurorum are rich in phenolic compounds and exhibit high antioxidant activity. These findings may contribute to a better understanding of the potential therapeutic uses of A. maurorum and could aid in the development of future plant-based medicines.

References

Al-Barham, M.B., Al-Jaber, H.I., Al-Qudah, M.A., Abu Zarga, M.H., 2017. New aristolochic acid and other chemical constituents of Aristolochia maurorum growing wild in Jordan. Natural Product Research, 31(3): 245–252.

Alı, Q., Kurubaş, M.S., Erkan, M., 2022. Biochemical composition and antioxidant activity of different types of tomatoes affected by ethylene treatment. Journal of Agricultural Sciences, 28(1): 8-15.

Anderson, K.J., Teuber, S.S., Gobeille, A., Cremin, P., Waterhouse, A.L., Steinberg, F.M., 2001. Walnut polyphenolics inhibit in vitro human plasma and LDL oxidation. The Journal of Nutrition, 131(11): 2837–2842.

Aytar, E.C., 2024. Antioxidant and antimicrobial properties of stachys maritima via quantum dots and molecular docking. Chemistry & Biodiversity, e202401057.

Binic, I., Lazarevic, V., Ljubenovic, M., Mojsa, J., Sokolovic, D., 2013. Skin ageing: Natural weapons and strategies. Evidence-Based Complementary and Alternative Medicine.

Bourhia, M., Laasri, F.E., Moussa, S.I., Ullah, R., Bari, A., Saeed Ali, S., Kaoutar, A., Haj Said, A.A., El Mzibri, M., Said, G., Khlil, N., Benbacer, L., 2019. Phytochemistry, antioxidant activity, antiproliferative effect, and acute toxicity testing of two moroccan aristolochia species. Evidence-Based Complementary and Alternative Medicine, 2019(1): 9710876.

Braca, A., De Tommasi, N., Di Bari, L., Pizza, C., Politi, M., Morelli, I., 2001. Antioxidant principles from bauhinia tarapotensis. Journal of Natural Products, 64(7): 892–895.

Chen, S.H., Chuang, Y.J,. 2002. Analysis of fatty acids by column liquid chromatography. Analytica Chimica Acta, 465(1–2): 145–155.

Das, T.S., Ramesh, L., Agastian, P., 2016. GC-MS analysis of bioactive constituents of Aristolochia bracteolata Linn with in-vitro antioxidant properties. Journal of Applied Pharmaceutical Science, 6(12): 061–066.

Dicson, S.M., Samuthirapandi, M., Govindaraju, A., Kasi, P.D., 2015. Evaluation of in vitro and in vivo safety profile of the Indian traditional medicinal plant Grewia tiliaefolia. Regulatory Toxicology and Pharmacology, 73(1): 241–247.

El, A., El Idrissi, Y., Khouchlaa, A., Bouyahya, A., Bakri, M’hamed Tijane, Y., 2020. Phytochemical characterization, in vitro antioxidant, cytotoxic, and antibacterial effects of Aristolochia longa L. Biointerface Research in Applied Chemistry, 11(1): 8129–8140.

Kalustian, P., 1985. Pharmaceutical and cosmetic uses of palm and lauric products. Journal of the American Oil Chemists’ Society, 62(2): 431–433.

Karaşin, N., Tolan, V., Haşimi, N., Demirci, Ö. 2019. Investigation of genotoxic, antimicrobial and antioxidant activities of leaf and flower extracts of Cynara syriaca Boiss. Journal of Agricultural Sciences, 25(3): 346-353.

Kolar, M.J., Konduri, S., Chang, T., Wang, H., McNerlin, C., Ohlsson, L., Härröd, M., Siegel, D., Saghatelian, A., 2019. Linoleic acid esters of hydroxy linoleic acids are anti-inflammatory lipids found in plants and mammals. Journal of Biological Chemistry, 294(27): 10698–10707.

Kuo, P.C., Li, Y.C., Wu, T.S.., 2012. Chemical constituents and pharmacology of the Aristolochia (馬兜鈴 mădōu ling) species. Journal of Traditional and Complementary Medicine, 2(4): 249–266.

López-Miranda, J., Badimon, L., Bonanome, A., Lairon, D., Kris-Etherton, P.M., Mata, P., Pérez-Jiménez, F., 2006. Monounsaturated fat and cardiovascular risk. Nutrition Reviews, 64: S2–S12.

Lou, S.N., Lin, Y.S., Hsu, Y.S., Chiu, E.M., Ho, C.T., 2014. Soluble and insoluble phenolic compounds and antioxidant activity of immature calamondin affected by solvents and heat treatment. Food Chemistry, 161: 246–253.

Mahmoudi, M., Boughalleb, F., Maaloul, S., Mabrouk, M., Abdellaoui, R., 2023. Phytochemical screening, antioxidant potential, and LC–ESI–MS profiling of ephedra alata and ephedra altissima seeds naturally growing in Tunisia. Applied Biochemistry and Biotechnology, 195(10): 5903–5915.

Okechukwu, P.N., 2020. Evaluatıon of antı-inflammatory, analgesıc, antıpyretıc effect of eıcosane, pentadecane, octacosane, and heneıcosane. Asian Journal of Pharmaceutical and Clinical Research, 29–35.

Osuna-Ruiz, I., López-Saiz, C.M., Burgos-Hernández, A., Velázquez, C., Nieves-Soto, M., Hurtado-Oliva, M.A., 2016. Antioxidant, antimutagenic and antiproliferative activities in selected seaweed species from Sinaloa, Mexico. Pharmaceutical Biology, 54(10): 2196–2210.

Pacheco, A.G., De Oliveira, P.M., Piló-Veloso, D., De Carvalho Alcântara, A.F., 2009. 13C-NMR data of diterpenes isolated from Aristolochia species. Molecules, 14(3): 1245-1262.

Porter, L.J., Hrstich, L.N., Chan, B.G., 1985. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry, 25(1): 223–230.

Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144–158.

Sivaraj, D., Shanmugam, S., Rajan, M., Sasidharan, S.P., Sathyanarayanan, S., Muniyandi, K., Thangaraj, P., de Souza Araújo, A.A., 2018. Evaluation of Aristolochia indica L. and Piper nigrum L. methanol extract against centipede Scolopendra moristans L. using Wistar albino rats and screening of bioactive compounds by high pressure liquid chromatography: a polyherbal formulation. Biomedicine & Pharmacotherapy, 97: 1603–1612.

Wagner, S.T., Hesse, L., Isnard, S., Samain, M.S., Bolin, J., Maass, E., Neinhuis, C., Rowe, N.P., Wanke, S., 2014. Major trends in stem anatomy and growth forms in the perianth-bearing Piperales, with special focus on Aristolochia. Annals of Botany, 113(7): 1139–1154.

Wang, J., Luo, T., Li, S., Zhao, J., 2012. The powerful applications of polyunsaturated fatty acids in improving the therapeutic efficacy of anticancer drugs. Expert Opinion on Drug Delivery, 9(1): 1–7.

Wanke, S., González, F., Neinhuis, C., 2006. Systematics of pipevines: combining morphological and fast‐evolving molecular characters to investigate the relationships within subfamily Aristolochioideae (Aristolochiaceae), International Journal of Plant Sciences, 167(6): 1215–1227.

Zhou, J., Chen, X., Cui, Y., Sun, W., Li, Y., Wang, Y., Song, J., Yao, H., 2017. Molecular structure and phylogenetic analyses of complete chloroplast genomes of two Aristolochia Medicinal Species. International Journal of Molecular Sciences, 18(9): 1839-1845.

Zhou, X.X., Jiang, G. Bin, Zhu, X.X., Liu, Z. Y., Huang, Y., Wang, G.T., Wang, R.J., 2019. Isotrema plagiostomum (Aristolochiaceae), a new species from Guangdong, South China. Phytotaxa, 405(4): 221-225.

Chemical Composition and Antioxidant Potential of Aristolochia maurorum Above-groud Extract: Phenolic and Phytochemical Analysis

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