Determination of Antioxidant Activities of Echinacea purpurea (L.) Moench. Plant Extracts Harvested at Different Times


Abstract views: 84 / PDF downloads: 38

Authors

DOI:

https://doi.org/10.5281/zenodo.13352969

Keywords:

Echinacea spp., phenolic content, dpph, purple cone flower, harvest time

Abstract

This research was carried out to determine the antioxidant activities of extracts obtained from plant parts of Echinacea purpurea L. (Moench) species harvested at different times. In the preparation of plantation, seeds for seedling production were planted in peat-containing pots on 24 April 2012. Rooted seedlings were planted in the experimental area in May. After planting, necessary cultural practices (irrigation, hoeing, etc.) of the plants were performed in 2013, 2014 and 2015. In this study, cone, leaf, flower and root parts of Echinacea plant harvested at different times between September 2014 and August 2015 were used. This study was conducted using the cone, leaf, flower, and root parts of the echinacea plant. While the cone, leaf and flower parts were harvested on 3 different dates, namely 24 July, 6 August and 19 August, the root part was harvested only once on 10 October. In this study, it was stated that the antioxidant activities of Echinacea purpurea species vary depending on the harvest period and the organs of the plant. The values of the highest total phenolic content and total flavonoid compound content were calculated. These values were determined as 59.107 mg GAE g-1 extract and 1807.286 mg QE g-1 extract, respectively. The lowest DPPH value was found to be 1.157 mg mL-1. These datas were found for the flower parts of the plant from the first harvest time, the leaf parts from the second harvest period and the flower parts from the second harvest date, respectively.

References

Açıkgöz, N.E., Gökçöl, A., 2004. Biyolojik Araştırmaların Bilgisayarda Değerlendirilmeleri. Ege Üniversitesi, Tohum Teknolojisi Araştırma ve Uygulama Merkezi, Yayın No:2, İzmir.

Amarowicz, R., Pegg, R., Rahimi-Moghaddam, P., Barl, B., Weil, J., 2004. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry, 84: 551–562.

Arvouet-Grand, A.B., Vennat, A., Pourrat, P., Legret., 1994. Standardisation extrait de propolis et identification des principaux constituants. Journal de Pharmacie de Belgique, 49: 462-468.

Awad, A., Fahim, H., Abd El-Ghany El-Shhat, Mahrose, K., Shazly, S., 2021. Dietary Echinacea purpurea administration enhanced egg laying performance, serum lipid profile, antioxidant status and semen quality in duck breeder during summer season. Journal of Animal Physiology and Animal Nutrition, 105: 757-765.

Billah, M.M., Hosen, M.B., Khan, F., Niaz, K., 2019. Echinacea, Nonvitamin and Nonmineral Nutritional Supplements. Elsevier, United Kingdom.

Cao, C., Kindscher, K., 2016. The Medicinal Chemistry of Echinacea Species, Echinacea. Springer, USA.

Chen, X.M., Hu, C., Raghubeer, E., Kitts, D.D., 2010. Effect of High Pressure Pasteurization on Bacterial Load and Bioactivity of Echinacea purpurea. Journal of Food Science, 75: 613–618.

Çalışkan, Ö., Odabaş, M.S., 2011. Ekinezya Türleri Genel Özellikleri ve Yetiştiriciliği. Anadolu Tarım Bilimleri Dergisi, 26:265-270.

Daniel, M., 2016. Medicinal Plants: Chemistry and Properties. CRC Press, United States.

Davis P.H., Mill, R.R., Kit, T., 1988. Flora of Turkey and the East Aegean Islands. Vol.10. Edinburgh: Edinburgh University Press.

Facino, R.M., Carini, M., Aldini, G., Saibene, L., Pietta, P., and Mauri, P., 1995. Echinacoside and caffeoyl conjugates protect collagen from free radical-induced degradation: a potential use of Echinacea extracts in the prevention of skin photodamage. Planta Medica, 61: 510–514.

Foster, S.,1991. Echinacea. Nature’s Immune Enhancer. Healing Art Press, Rochester, USA.

Gezer, K., Duru, M.E. Kıvrak, I., Türkoğlu, A., Mercan, N., Türkoğlu, H., Gülcan, S., 2006. Free-radical scavenging capacity and antimicrobial activity of wild edible mushroom of Turkey. African Journal of Biyotechnology, 5(20):1924-1928.

Hobbs, C., 1994. A litarature review; botany, history, chemistry, pharmacology, toxicology and clinical uses. Herbal Gram, 33-41.

Hu, C., Kitts, D., 2000. Studies on the antioxidant activity of Echinacea root extract. Journal of Agricultural and Food Chemistry, 48: 1466–1472.

Jahanian, E., Jahanian, R., Rahmani, H., Alikhani, M., 2017. Dietary supplementation of Echinacea purpurea powder improved performance, serum lipid profile, and yolk oxidative stability in laying hens. Journal of Applied Animal Research, 45: 45–51.

Jamshidi-Kia, F., Lorigooini, Z., Amini-Khoei, H., 2018. Medicinal plants: past history and future perspective. Journal of Herbmed Pharmacology, 7(1):1-7.

Jukić, H.A., Habeš, S.B., Aldžić, A.A., Durgo, K.C., Kosalec, I.D., 2015. Antioxidant and prooxidant activities of phenolic compounds of the extracts of Echinacea purpurea (L.). Bulletin of the Chemists and Technologists of Bosnia and Herzegovina, 44: 43-52.

Kan, Y., 2011. Türkiye’de Tıbbi ve Aromatik Bitkiler Tohumculuk Politikaları. Türkiye IV. Tohumculuk Kongresi, Kongre Bildiriler Kitabı, 14-17 Haziran, Samsun s.348.

Karaca Öner, E., Arslanoğlu, Ş.F., İskender, R., 2023. Determination of the effects of different nitrogen and phosphorus applications on leaf area of echinacea (Echinacea purpurea L.). ISPEC Journal of Agricultural Sciences, 7(1): 53-59.

Kobylinska, L.I., Tymochko, M.F., 2000. The role of prooxidant-antioxidant balance in the adaptational reactions of organism. Experimental and Clinical Physiology and Biochemistry, 4: 52-58.

Lee, J., Scagel, C.F., 2009. Cichoric acid and found in basil (Ocimum basilicum) leaves. Food Chemistry, 115: 650-656.

Lee, T.T., Chen, C.L., Shieh, Z.H., Lin, J.C., Yu, B., 2009. Study on antioxidant activity of Echinacea purpurea L. extracts and its impact on cell viability. African Journal of Biotechnology, 8(19): 5097-5105.

Mat, A., 2002. Echinaceae Türleri. 14. Bitkisel İlaç Hammaddeleri Toplantısı, Kongre Bildiriler Kitabı, 29-31 Mayıs, Eskişehir, s.59-67.

Merali, S., Binns, S., Paulin-Levasseur, M., Ficker, C., Smith, M., Baum, B., Brovelli, E., Arnason, J., 2003. Antifungal and antiinflammatory activity of the genus Echinacea. Pharmaceutical Biology, 41: 412–420.

Mistrikova, I., Vaverkova, S., 2007. Morphology and anatomy of echinaceae purpurea, Echinaceae angustifolia, Echinaceae pallida and Parthenium integrifolium. Biologia, 62(1): 2-5.

Schober, P., Boer, C., Schwarte, L.A., 2018. Correlation Coefficients: Appropriate Use and Interpretation. Anesthesia & Analgesia, 126:1763-1768.

Razic S., Onija, A., Potkonjak, P. 2003. Trace element analysis of E. purpurea-herbal medicine. Journal of Pharmaceutical and Biomedical Analysis, 33:845-850.

Shrestha, P.M., Dhillion, S.S., 2006. Diversity and traditional knowledge concerning wild food species in a locally managed forest in Nepal. Agroforestry Systems, 66: 55–63.

Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 299: 152-178.

Smith, T., May, G., Eckl, V., Reynolds, C.M., 2020. US sales of herbal supplements increase by 8.6 % in 2019. Herbal Gram, 54–69.

Stanisavljevic, I., Stojicevic, S., Velickovic, D., Veljkovic, V., Lazic, M., 2009. Antioxidant and antimicrobial activities of echinacea (Echinacea purpurea L.) tinctures obtained by classical and ultrasound extraction. Chinese Journal of Chemical Engineering, 17(3): 478-483.

Şenkal, B.C., 2020. The Role of secondary metabolites obtained from medicinal and aromatic plants in our lives ISPEC Journal of Agricultural Sciences, 4(4): 1071-1079.

Upton, R., Graff, A., 2007. Echinaceae purpurea root standarts of analysis, quality control and therapeutics. American Herbal Pharmacopoeia, 1-61.

Yezerska, O., Hudz, N., Kobylinska, L., Filipska, A., Turkina, V., Brindza, J., Wieczorek, P.P., 2022. Methodical approach to the determination of the antioxidant activity of the Echinacea purpurea and Monarda fistulosa tinctures as a quality criterion of the development of their composition and laboratory technology. Acta Medica Leopoliensia, 28(1-2): 89-98.

Downloads

Published

2024-12-01

How to Cite

ŞAHİN, R. V., & COŞGE ŞENKAL , B. . (2024). Determination of Antioxidant Activities of Echinacea purpurea (L.) Moench. Plant Extracts Harvested at Different Times. ISPEC Journal of Agricultural Sciences, 8(4), 926–937. https://doi.org/10.5281/zenodo.13352969

Issue

Section

Articles