The Effect of Melatonine Applications on Some Morphological Charaters of Cucumber (Cucumis sativus L.) Seeds with and without Chilling Stress


Abstract views: 434 / PDF downloads: 338

Authors

  • Lütfullah BAŞLAK Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü
  • Özlem ÜZAL Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü
  • Fikret YAŞAR Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü

DOI:

https://doi.org/10.46291/ISPECJASvol5iss2pp350-361

Keywords:

Cucumis sativus, cucumber, chilling stres, , tolerance, melatonin

Abstract

In the research using Beith F1 cucumber variety; In the research; The effects of chill and foliar applied melatonin on some morphological characteristics of seedlings were aimed to be investigated. Seedlings with 3-4 true leaves were chilled. For seedlings applied to the cold, distilled water containing 0, 1, 10, 20, 30 and 40 μM melatonin was sprayed onto the leaves of the plants. One full day after melatonin application, half of the plants were exposed to cold stress in the climate cabinet for 15 days, and the other half were kept in the climate room under normal conditions (25/20 °C light / dark). Samples were taken after the plants exposed to cold stress were kept in the incubator for 15 days at 5 ± 1 °C dark (for 12 hours) and 10 ± 1 °C light (for 12 hours). Some basic growth parameters of plants were measured and some biochemical analyzes were done. When the plant growth parameters were evaluated, it was determined that 30 and 40 µM melatonin treated plants limited the plant growth. In addition, plants with no melatonin applied had the highest scale value, whereas melatonin applications from the leaf were observed to be effective in reducing visual damage, and the least visual damage was observed in the application of 40 µM melatonin. As a result, it is thought that melatonin applications may be a physiologically effective adjuvant that can have a positive effect in reducing harmful effects caused by chilling stress.

References

Anonim, 2020 http://tarimsalistatistik.com/tr-TR/Sayfa/hiyar-yetistiriciligi. (Erişim tarihi 12.05.2020).

Arnao, MB. 2014. Phytomelatonin: Discovery, Content, and Role in Plants. Advances in Botany, e815769. doi:10.1155/2014/815769.

Arnao, MB., Hernández-Ruiz, J. 2018. Melatonin in its relationship to plant hormones. Ann. Bot., 121, 195–207.

Arnao MB., Hernandez-Ruiz J. 2009. Protective Effect of melatonin Against Chlorophyll Degradation During the Senescence of Barley Leaves. Journal of Pineal Research, 46 (1):58-63.

Arnao MB, Hernandez-Ruiz J. 2013. Growth Conditions Determine Different Melatonin Levels in Lupinus albusL. Journal of Pineal Research, 55: 149–155. 136.

Arnao, M. B., Hernandez- Ruiz, J. 2017. Growth activity rooting capacity and tropism: three auxinic precepts fulfilled by melatonin. Acta Physiol Plant 39127.

Aguilera Y, Herrera T, Liébana R. 2015. Impact of melatonin enrichment during germination of legumes on bioactive compounds and antioxidant activity. Journal of Agricultural and Food Chemistry 63: 7967–7974.

Bajwa VS, Shukla MR, Sherif SM, Murch SJ, Saxena PK. 2014. Role of Melatonin in Alleviating Cold Stress in Arabidopsis thaliana. Journal of Pineal Research, 56: 238–245.

Batu, A., Thompson, AK., Ghafır, SAM., Rahman, NAA, 1997. Minolta ve hunter renk ölçüm aletleri ile domates, elma ve muzun renk değerlerinin karşılaştırılması, Gıda, 22 (4), 301-307.

Byeon, Y., Park, S., Yool Lee, H., Kim, YK., Back. K., Elevated productionof melatonin in transgenic rice seeds expressing ricetryptophan decarboxylase. Journal Pineal Resarch 2014;56:275–282.

Chen, G., Huo, Y., Tan, D.X., Liang, Z., Zhang, W., Zhang, Y. 2003. Melatonin in Chinese Medicinal Herbs., 73: 19–26.

Dubbels, R., Reiter, R.J., Klenke, E., Goebel, A., Schnakenberg, E., Ehlers, C. 1995. Melatonin in Edible Plants Identified by Radioimmunoassay and by High Performance Liquid Chromatography Mass Spectrometry. Journal of Pineal Research, 18: 28– 31.

Hattori, A, Migitaka, H., Masayaki, I., Itoh, M., Yamamoto, K., Ohtani-Kaneko, R., Hara, M., Suzuki, T., Reiter, R.J. 1995. Identification of Melatonin in Plant Seed Its Effects on Plasma Melatonin Levels and Binding to Melatonin Receptors in Vertebrates. International Journal of Biochemistry and Molecular Biology, 35: 627–634.

Han QH., Huang B., Ding CB. 2017. Effects of melatonin on anti-oxidative systems and Photosystem II in cold-stressed rice seedlings. Frontiers in Plant Science 8: 785.

Korkmaz A., Değer Ö., Cuci Y. 2014. Profiling the Melatonin Content in Organs of the Pepper Plant during Different Growth Stages. Scientia Horticulturae, 172: 242–247.

Korkmaz, A. 2002. Amelioration of Chilling Injuries in Watermelon Seedlings by Abscisic Acid. Turkish Journal of Agriculture and Forestry. 26: 17-20.

Korkmaz, A., Demir, Ö., Kocaçınar, F., Yakup, 2016. Biber Fidelerinde Yapraktan Yapılan Melatonin Uygulamalarıyla Üşüme Stresine Karşı Toleransın Arttırılması. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 19(3): 348-354.

Korkmaz, A., Karaca, A., Kocaçınar, F., Cuci, Y. 2017. The effect of seed treatment with melatonin on germination and emergence performance of pepper seeds under chilling stress. Tarım Bilimleri Dergisi/Journal of Agricultural Sciences, 23 (2): 167-176

Lerner, AB., Case, JD., Takahashi, Y. 1958. Isolation of Melatonin, a Pineal Factor That Lightness Melanocytes. Journal of American Chemical Society, 80: 2587-2591.

Li, C., Wang, P., Wei, Z., Liang, D., Liu, C., Yin, L., Jia, D., Fu, M., Ma, F. 2012. The Mitigation Effects of Exogenous Melatonin on Salinity-Induced Stress in Malus hupehensis. Journal of Pineal Research, 53: 298-306.

Liu, J., Wang, W., Wang, L., Sun, Y. 2015. Exogenous Melatonin improves Seedling Health Index and Drought Tolerance in Tomato. Plant Growth Regulation, 77: 317–326.

Posmyk, MM., Janas, KM. 2009. Melatonin in Plants. Acta Physiologia Plantarum, 31: 1–11.

Reiter, RJ. 1991. Pineal Melatonin: Cell Biology of Its Physiological Interactions. Endocrine Reviews, 12: 151–181.

Reiter, RJ., Tan, DX., Zhou, Z., Cruz, MHC, Fuentes-Broto, L, Galano, A. 2015. Phytomelatonin: Assisting Plants to Survive and Thrive. Molecules, 20: 7396-7437.

Solak, FT. 2016.Çanakkale şartlarında tarla ve tünel altında kıvırcık salata (Lactuca sativa var. crispa) yetiştirme olanakları, Selçuk Üniversitesi Fen Bilimleri Enstitüsü.

Tal O., Haim A., Harel O., Gerchman Y. 2011. Melatonin as an Antioxidant and Its Semi-Lunar Rhythm in Green Macroalga Ulvasp. Journal of Experimental Botany,62: 1903–1910

Tan DX, Hardeland R, Manchester LC, Korkmaz A, Ma S, Rosales-Corral S., Reiter RJ. (2012). Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science. Journal of Experimental Botany 63: 577-597

Tuğa, H., Üzal, Ö. "Bazı Organik Materyallerin Kıvırcık Yaprak Salata (Lactuca Sativa Var. Crispa)’ Nın Morfolojik Özellikleri Üzerine Etkisinin Araştırılması," II. International Scientific and Vocational Studies Congress (Bilmes 2018) , Nevşehir, Turkey, pp.1177-1181, 2018

Xu, SC., Li, YP., Hu, J., Guan, YJ., Ma, WG., Zheng, YY., Zhu, SJ. 2010. Responses of Antioxidant Enzymes to Chilling Stress in Tobacco Seedlings. Agricultural Sciences in China, 9: 1594-1601.

Wen D., Gong B., Sun S. 2016. Promoting roles of melatonin in adventi-tious root development of Solanum lycopersicum L. by regulating auxin and nitric oxide signaling. Frontiers in Plant Science 7: 718.

Zhang R., Sun Y., Liu Z., Jin W., Sun Y. 2017. Effects of melatonin on seedling growth, mineral nutrition, and nitrogen metabolism in cucumber under nitrate stress. Journal of Pineal Research 62: e12403.

Zorlugenç, FK., Fenercioğlu, H., 2012, Ozmotik dehidrasyon uygulamasının trabzon hurması meyvelerinin kuruma davranışı ve ürün kalitesi üzerine etkileri, Ç.Ü Fen ve Mühendislik Bilimleri Dergisi, 28(5), 149-159.

Published

2021-06-02

How to Cite

Lütfullah BAŞLAK, Özlem ÜZAL, & Fikret YAŞAR. (2021). The Effect of Melatonine Applications on Some Morphological Charaters of Cucumber (Cucumis sativus L.) Seeds with and without Chilling Stress. ISPEC Journal of Agricultural Sciences, 5(2), 350–361. https://doi.org/10.46291/ISPECJASvol5iss2pp350-361

Issue

Section

Articles