Effects of Various Levels of Luteinizing Hormone and Caprine Follicular Fluid on In Vitro Embryo Production of Shami Goat
Abstract views: 568
/ 
PDF downloads: 210
DOI:
https://doi.org/10.46291/ISPECJASvol5iss3pp575-584Keywords:
follicular fluid, goat, luteinizing hormone, oocytes.Abstract
In the current study, the hypothesis of the effects of luteinizing hormone (LH) and follicular fluid (FF) derived from follicles of varying size on in vitro embryo production of the Shami goat breed were tested. The caprine follicular fluid (cFF) was obtained from healthy female’s ovaries by aspiration method and classified into two main classes (follicles with a diameter of ≤ 2mm and ≥3mm). The resulting cFF was added to the culture medium TCM-199 through six Treatments (A, B and C with a source of follicle size of ≤ 2mm; D, E and F with a source of size of ≥3mm). LH was added only to four of the previous Treatments with the levels of 50 µg ml-1 (B and E) and100 µg ml-1 (C and F). Results of the study showed that the oocytes incubated in Treatment F achieved a clear superiority (p=0.001) in the rates of maturation (87.0%), fertilization (80.0%) and cleavage (82.3%). The oocytes incubated in the same Treatment (F) continued to outperform (p= 0.006) by achieving the best rates across cleavage stages at 2-16 cell (16%; the lower value of arrest) and blastocyst (42%). Significant differences (P=0.03) were observed among the rates of Type 1embryos (the highest rate: 45.3%; Treatment F) and Type 3 embryos (the highest rate: 45.1%; Treatment A). No significant differences were observed in the rates of morula and Type 2 embryos. It is advised to add 15% of the cFF derived from follicles with a diameter of ≥3mm and 100 µg of LH ml-1 in the maturation media to obtain higher rates of maturation and cleavage of goat oocytes.
References
Accardo, C., Dattena, M., Pilichi, S., Mara, L., Chessa, B., Cappai, P. 2004. Effect of recombinant human FSH and LH on in vitro maturation of sheep oocytes; embryo development and viability. Animal Reproduction Science, 81(1-2): 77–86.
Ashworth, C.J., Toma, L.M., Hunter, M.G. 2009. Nutritional effects on oocyte and embryo development in mammals: implications for reproductive efficiency and environmental sustainability. Philos Trans R Soc B Biol Sci, 364: 3351–3361.
Barakat, I.A.H., Kandeal, S.A., El–Ashmaoui, H.M., Barkawi, A., EL-Nahass, E. 2012. Effect of medium type and luteinizing hormone (LH) on in vitro maturation of Egyptian buffalo oocytes. African Journal of Biotechnology, 11(20): 4620-4630.
Chapuis, A., Gala, A., Ferrières-Hoa, A., Mullet, T., Bringer-Deutsch, S., Vintejoux, E., Torre, A., Hamamah, S. 2017. Sperm quality and paternal age: effect on blastocyst formation and pregnancy rates. Basic Clin Androl, 27: 1–9.
Chundekkad, P., Błaszczyk, B., Stankiewicz, T. 2020. Embryonic mortality in sheep: a review. Turkish J. Veterianry Anim Sci, 44: 167–173.
Conti, M., Hsieh, M., Zamah, A.M., Oh, J.S.2012. Novel signaling mechanisms in the ovary during oocyte maturation and ovulation. Mol Cell Endocrinol, 356: 65–73.
Da Broi, M.G., Giorgi, V., Wang, F., Keefe, D.L., Albertini, D., Navarro, P.A. 2018. Influence of follicular fluid and cumulus cells on oocyte quality: clinical implications. Journal of assisted reproduction and genetics, 35(5): 735–751.
De Oliveira Bezerra, A., Nicacio, A.C., de Oliveira Menezes, G.R., da Costa Gomes, R., da Silva, L.O.C., de Souza Rocha-Frigoni, N.A., Mingoti, G.Z., da Silva Leão, B.C., da Costa e Silva, E.V., Nogueira, É. 2019. Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves. Animal Reproduction Science, 208: 106101.
Dell’Aquila, M.E., Cho, Y.S., Minoia, P., Traina, V., Lacalandra, G. M., Maritato, F. 1997. Effects of follicular fluid supplementation of in-vitro maturation medium on the fertilization and development of equine oocytes after in- vitro fertilization or intracytoplasmic sperm injection. Human Reproduction, 12(12): 2766–2772.
Dinopoulou, V., Drakakis, P., Kefala, S., Kiapekou, E., Bletsa, R., Anagnostou, E., Kallianidis, K., Loutradis, D. 2016. Effect of recombinant-LH and hCG in the absence of FSH on in vitro maturation (IVM) fertilization and early embryonic development of mouse germinal vesicle (GV)-stage oocytes. Reproductive Biology, 16(2): 138–146.
Dos Santos-Neto, P.C., Vilariño, M., Cuadro, F., Barrera, N., Crispo, M., Menchaca, A. 2020. Cumulus cells during in vitro fertilization and oocyte vitrification in sheep: Remove, maintain or add?. Cryobiology, 92: 161-167.
Ervandi, M., Susilawati, T., Wahyuningsih, S. 2013. Pengaruh pengencer yang berbeda terhadap kualitas spermatozoa sapi hasil sexing dengan rradien albumin (putih telur). JITV, 18: 177–184.
Fayad, T., Levesque, V., Sirois, J., Silversides, D.W., Lussier, J.G. 2004. Gene expression profiling of differentially expressed genes in granulosa cells of bovine dominant follicles using suppression subtractive hybridization. Biol Reprod, 70: 523–533.
Filicori, M., Santoro, N., Merriam, G.R., Crowley, W.F.J. 1986. Characterization of the physiological pattern of episodic gonadotropin secretion throughout the human menstrual cycle. J. Clin. Endocrinol. Metab, 62: 1136-1144.
Ginther, O.J., Beg, M.A., Bergfelt, D. R., Donadeu, F.X., Kot, K. 2001. Follicle selection in monovular species. Biology of Reproduction, 65(3): 638–647.
Harper, K.M., Brackett, B.G. 1993. Bovine blastocyst development after in vitro maturation in a defined medium with epidermal growth factor and low concentrations of gonadotropins. Biol. Reprod, 48: 409-416.
Ikeda, S., Azuma, T., Hashimoto, S., Yamada, M.1999. In vitro maturation of bovine oocytes with fractions of bovine follicular fluid separated by heparin affinity chromatography. Journal of Reproduction and Development, 45(6): 397–404.
Jaffe, L.A., Egbert, J.R. 2017. Regulation of mammalian oocyte meiosis by intercellular communication within the ovarian follicle. Annu Rev Physiol, 79: 237–260.
Kim, K.S., Tajima, J., Yamaji, T., Mitsumizo, N., Fujita, K., Cho, H.J., Kato, H., Utsumi, K. 1993. Effects of supplemented follicular fluid to IVM serum tree-medium and developmental of bovine oocytes. Jap Sot Anim Reprod Tech, 15:41-48.
Kusumaningrum, D.A., Purwantara, B., Yusuf, T.L., Situmorang, P. 2015. Microencapsulation of bovine spermatozoa: Cryopreservation microencapsulation of sperm using glycerol. JITV, 20: 233–241.
Leroy, J.L.M., Vanholder, T., Delanghe, J., Opsomer, G., Van Soom, A., Bols, P.E., de Kruif, A. 2004. Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cows. Animal Reproduction Science, 80:(3-4): 201–211.
Mardenli, O. 2020. Role of follicle size, igf-i, glucose and hormones on nuclear maturation events of awassi sheep oocytes (Ovis aries). ISPEC Journal of Agr. Sciences, 4(4): 732-746
Mehlmann, L.M. 2005. Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation. Reproduction, 130: 791–799.
Mihm, M., Baker, P.J., Ireland, J.L., Smith, G.W., Coussens, P.M., Evans, A.C., Ireland, J.J. 2006. Molecular evidence that growth of dominant follicles involves a reduction in follicle-stimulating hormone dependence and an increase in luteinizing hormone dependence in cattle. Biol Reprod, 74: 1051–1059.
Naito, K., Fukuda, Y., Ishibashi, I. 1989. Developmental ability of porcine ova matured in porcine follicular fluid in vitro and fertilized in vitro. Theriogenology, 31:1049-1057.
Naito, K., Fukuda, Y., Toyoda, Y. 1988. Effect of porcine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gam Res, 21: 289-295.
Nedresky, D., Singh, G. 2020. Physiology, Luteinizing Hormone. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing,: 30969514.
SAS Institute Inc. 2017. SAS/STAT® 14.3 User’s Guide. Cary, NC: SAS Institute.
Silva, D.S., Pereira, L.P., Navarro, R.B., Rosa, D.C., Pessoa, G.A., Silva, C.A.M., Rubin, M.I.B. 2010. In vitro production of Bos Taurus indicus embryos with cysteamine. Anim. Reprod, 7(1): 29-34.
Tripathi, S.K., Farman, M., Nandi, S., Girish Kumar, V., Gupta, P.S.P. 2015. Biochemical constituents of ovarian follicular fluid in ruminants and their significance in follicle and oocyte development. J Vet Sci Med Diagn, 4(3):2-7.
Twigg-Flesner, A., Newman, A., Davies, E. 2014. The Effect of Mare Age and Reproductive Status on Embryo Recovery Rate and the Quality of Embryos Recovered. In: Br Soc Anim Sci Conf 2014. United Kingdom (UK).
Vajta, G., Rindom, N., Peura, T.T., Holm, P., Greve, T., Callesen, H. 1999. The effect of media, serum and temperature on in vitro survival of bovine blastocysts after Open Pulled Straw (OPS) vitrification. Theriogenology, 52: 939–948.
Webb, R., Campbell, B.K. 2007. Development of the dominant follicle: mechanisms of selection and maintenance of oocyte quality. Soc Reprod Fertil Suppl, 64: 141-63.
Wen, X., Tozer, A., Butler, S., Bell, C., Docherty, S., Iles, R. 2006. Follicular fluid levels of inhibin A, inhibin B, and activin A levels reflect changes in follicle size but are not independent markers of the oocyte’s ability to fertilize. Fertility and Sterility, 85(6): 1723–1729.
Wintner, E.M., Hershko-Klement, A., Tzadikevitch, K., Ghetler, Y., Gonen, O., Wintner, O., Shulman, A., Wiser, A. 2017. Does the transfer of a poor-quality embryo together with a good quality embryo affect the In Vitro Fertilization (IVF) outcome? J Ovarian Res, 10:2.
Zuelke, K.A,, Brackett, B.G. 1992. Effects of luteinizing hormone on glucose metabolism in cumulus-enclosed bovine oocytes matured in vitro. Endocrinology, 131(6): 2690-2696.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 The copyright of the published article belongs to its author.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
